GE Ettan IPGphor 3, Instrument User Manual

Page: 39 / 64

Operating instructions
3
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
39
Editing protocol parameters
Edit the Rehydration parameters, time and temperature, and the IEF parameters,
temperature, µA/Strip and number of strips as necessary.
Note: The cursor stops only in the fields that can be edited. Once the cursor is in
position, press the
up or down arrow keys to adjust the value.
The available time range is 0–99:59 h, with 10 min resolution. (Hence, only the first
three digits are editable). A minimum of 10 h is required for the rehydration of IPG
strip gels, but overnight is recommended. This protocol step can also be used as
a simple timer to start the protocol at a convenient time. If the IPG strips are
already rehydrated and ready for immediate focusing, set the rehydration
duration to 00:00 Hrs.
To adjust the rehydration temperature :
1 use the left and right arrow keys to position the cursor and
2 the up and down arrow keys to select the value within the range of 15–30 °C.
The original factory programmed value is 20 °C.
Note: Maintaining the actual platform temperature at <18 °C or >25 °C may
require placement of the Ettan IPGphor 3 unit in a location of lower or
higher than ambient temperature (e.g. a cold room).
It may be necessary to operate the instrument in an air conditioned room
to prevent condensation from collecting on the platform in humid
environments when the platform is run at greater than 7 °C below ambient
temperature.
The third line contains fields to set the platform temperature and current limit for
all isoelectric focusing steps:
P r o t # 1 F i l e 1
R e h y d r a t e 1 0 : 0 0 H r @ 2 0
˚
C
I E F @ 2 0
˚
C 5 0
µ
A / S t r i p
1 S t r i p s 0 S t e p s
P r o t # 1 F i l e 1
R e h y d r a t e 1 0 : 0 0 H r @ 2 0
˚
C
I E F @ 2 0
˚
C 5 0
µ
A / S t r i p
1 S t r i p s 0 S t e p s