GE Ettan IPGphor 3, Instrument User Manual

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Operating instructions
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Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
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To toggle between VhT, VhS and Hrs:
1 move the cursor to the Hrs/Vhrs field of the display using the right or left
arrow key, and
2 change the display to reflect VhT, VhS and Hrs using the up and down keys.
To change the display to show remaining Hrs, VhS or VhT:
1 move the cursor under the Elapsed or ~ Remain field and use the up or down
buttons.
The temperature, voltage and current limits for the current step may be adjusted
directly on this screen as well.
Pressing the Edit key will access the step parameter screen. Pressing Edit again
will return to the Running screen. Use the up arrow in the S1 field to access the
Rehydration/IEF screen.
Note: If you adjust the voltage during a gradient step the step will be converted
to Step for the remainder of the step.
As isoelectric focusing proceeds, the bromophenol blue tracking dye will migrate
toward the anode and leave the IPG strip. This generally occurs well before
focusing is complete.
Note: A colorless strip is no indication that IEF is complete.
If the tracking dye does not move, then no current is flowing across the strip.
Check that the strip holder electrodes are in contact with the electrode areas. See
the Troubleshooting section for additional suggestions.
When the protocol ends, audible beeps will sound and the following screen is
displayed:
The final instrument values are displayed. Press the STOP key, as indicated, to
reset the instrument.
R u n n i n g P r o t #
S 1 G r a d 5 0 0 V 1 : 0 0 H r s
S 2 G r a d 2 0 0 0 V 1 : 0 0 H r s
S 3 S t e p 8 0 0 0 V 2 : 0 0 H r s
R u n e n d e d a t
8 0 0 0 V 1 2 0 0 0 V h r s To t a l
5 0
µ
A 8 : 0 0 H r To t a l
P r e s s S t o p t o r e s e t