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ROCK IMAGER User's Guide | Chapter 10: Configuring FRAP Imaging Settings
General FRAP options include settings related to the curve generated based on the FRAP data.
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Single Component Fitting.
Fits the pixel intensity recovery data with one Bessel function.
The result of this numerical fitting is a single diffusion rate. This method is accurate enough
for most applications, if protein molecules are labeled with dye.
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Double Component Fitting.
Fits the pixel intensity recovery data with two Bessel functions.
The result of this numerical fit is two distinct diffusion rates. This method is useful if the
protein is not purified well. Some small molecule lipids are labeled with dye, which generate
the two distinct diffusion rates: diffusion rate of lipid and diffusion rate of protein.
General Options