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ROCK IMAGER User's Guide | Chapter 6: Imaging Methods
How Does MFI work?
MFI’s optics are optimized for both UV and visible fluorescence imaging to provide high-contrast
images. For example, the UV LED and condenser lenses are positioned to maximize the intensity of
the UV illumination in order to boost signal strength and produce high-quality UV images.
ROCK IMAGER MFI comes with three different filter modules capable of imaging your crystal with
different excitation and emission wavelengths. The standard package includes one 290 nm
excitation module for UV imaging, and two visible fluorescence filter sets for detecting the
fluorescence from Texas Red and Fluorescein dyes.
If you would like to image fluorescence from other dyes and install a different filter module,
contact
.
MFI Schematic Drawing
Finding protein-protein complexes
With MFI, you can easily differentiate between crystals of a protein-protein complex and crystals of
just one protein.