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ROCK IMAGER User's Guide | Chapter 6: Imaging Methods
3. Choose from the following options:
●
To image an entire plate with the MFI imaging settings: in the
Imager
menu, point to
Image
, and click
Plate(s)
.
●
To image an entire plate with the imaging setting(s) of choice: in the
Imager
menu,
point to
Image
, and click
Plate(s) Using
. ROCK IMAGER will display a dialog box that
asks you to select available imaging settings.
●
To inspect your plates well-by-well or drop-by-drop without taking images, go to the
Live Image
tab and use the manual navigation controls.
●
To image a specific drop with the MFI imaging settings: in the
Imager
menu, point to
Image
, and click
Drop
.
●
To image a specific drop with your choice of imaging settings: in the
Imager
menu,
point to
Image
, and click
Drop Using
.
●
If you have tagged certain drops as interesting in ROCK MAKER, you can opt to image
only those drops by going to the
Imager
menu and selecting
Interesting Drop(s)
in
the
Image
menu to use the default imaging setting.
OR
Point to
Image
, and click
Interesting Drop(s) Using
to use a custom imaging setting.
4. When your plates have been imaged at least once, you can view the images in ROCK
MAKER.
●
As long as ROCK IMAGER Processor is running, you should see thumbnails of your
images on the Canvas.
If your images are not showing up, try pressing
F5
on your keyboard while in ROCK
MAKER to refresh the image display.
Tip:
For imaging with ROCK IMAGER 1000 and ROCK IMAGER 2 MFI, use the
following Camera Control and Optics Control settings as a starting point:
●
Exposure:
50 ms. You can also click the
Auto
button
next to
the arrow buttons in the
Exposure
box to automatically adjust the
exposure.
●
Gain:
5.
●
Brightness:
100%.
