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ROCK IMAGER User's Guide | Appendix A: Optics Controls
This table explains how to use each of the optics controls, which are used during manual inspections.
See
on page 60 for more information.
Control
Imaging Methods
Description
Zoom
●
Fluorescence
●
SONICC
Available zoom settings depend on the options you purchased.
In most cases the zoom selection will cause a mechanical change
in the lens, which produces the desired zoom.
Digital zoom with fixed objectives is available in all ROCK
IMAGERs with nine megapixel cameras.
In SONICC, changing the zoom value changes the laser beam
scan angle.
In MFI, the
Zoom
field controls the continuous zoom, which is
achieved by cropping the images captured by the camera. The
FOV for fluorescent images will match the FOV for visible images,
which also enhances the viewing of images in ROCK MAKER.
Field of View (FOV)
●
Visible
●
SLP Visible
●
UV
●
Fluorescence
●
FRAP
●
SONICC
You can use the
FOV
fields to manually set the size, in µm, of the
image you would like to view. As you change the FOV value using
either the text entry field or the arrows, the zoom value
automatically adjusts.
Sensor
●
SONICC
The
Sensor
selection determines whether you are using SHG or
UV-TPEF.
The SHG imaging method images the sample with IR and detects
the SHG (532 nm) in transmission. Signal is only generated from
chiral crystals.
The UV-TPEF imaging method images the sample with green
light (532 nm) and detects the two photon excited fluorescence
generated (350-400 nm). Signal is generated from UV-
fluorescent molecules like tryptophan.
Brightness
●
Fluorescence
The
Brightness
values range from 0% - 100%. For imaging with
ROCK IMAGER 1000 and ROCK IMAGER 2 MFI, set the
Brightness
to
100%
as a starting point.
