53
Prerequisites
●
You have loaded and scanned the plate.
To view a plate's automatic imaging information:
●
To view a plate's imaging schedule, go to the
Imager
tab and click
More
on
Plate Information
.
Alternatively, right-click a plate on the
Scheduling
tab and select
Plate Information
.
During High-Throughput FRAP imaging, pre-bleach and post-bleach images are taken for each
well, and then the stage positions the A1 drop back under the camera for end-state imaging. As
FRAP results are calculated by analyzing the intensity change of the bleached area, and bleach
spots are ~15 microns wide, an image alignment algorithm is used to account for the fact that the
stage position is not repeatable from pre-bleach to end-state positioning on the same drop within
10 microns. The software algorithm repositions the end-state image to correctly overlay with the
pre-bleach image. Expected values are approximately 10-30 µm. Image offsets between the pre-
bleach and post-bleach images are also calculated. However, very minimal changes are expected
here as the stage does not reposition in between the acquisition of these images.
Starting with ROCK IMAGER 2.16, these offset numbers are available on the Imaging Settings panel
in ROCK MAKER. These values are displayed for troubleshooting purposes.
Viewing FRAP offset values:
1. Using ROCK MAKER, locate the FRAP experiment in the
Explorer
and double-click it to
open it.
2. Click the
Canvas
tab.
3. Double-click one of the drops. The drop image will appear in the
Drop
tab.
Interpreting FRAP Offset Information
Drop Tab in ROCK MAKER
ROCK IMAGER User's Guide | Chapter 9: Using RI 2, FRAP Benchtop & SONICC Benchtop
