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NT8 User's Guide | Chapter 13: LCP Experiments
●
Once in place, tighten the plunger lock thumbscrew (located directly above the
plunger lock).
5.
Use the lever to dispense a small amount of LCP sample from the syringe to prevent
clogging and remove any air from the needle. Wipe the tip of the LCP syringe with a
chem wipe.
6.
The sensors on the NT8 will detect the presence of the LCP syringe. A dialog box will
open asking if you want to calibrate the syringe. Click
Yes
.
7.
Select the size of your LCP syringe and click
OK
. The NT8 will start calibrating the LCP
syringe.
Option 1: Set Up the Software Using the LCP Template
An LCP dispense template is preinstalled with your software. You may use it if you plan to
combine 800 nL well solution with 50 nL LCP drops. You must also use the MRC 2 Well
Crystallization plate as your well solution source.
To use this template:
1.
Click the
Open Task List
button .
2.
Open the Sample folder and select the
LCP 50 nL Monolein And 800 nL Well
Solutio
n task list.
3.
Click
Open
.
4.
Run your experiment by clicking the
Run Task List
button .
Option 2: Manually Set Up the Software
1.
Drag the LV Tip Caddy from the Plate Type library to LV Tip Caddy station image.
2.
Drag the LCP Plate Adapter from the Plate Type library to Microplate Station 1.
3.
Find the plate type you used for your well solution and drag the plate to Microplate
Station 2.
4.
Add the
Pick Tip
command to the Task List by double-clicking the LV Tip Caddy
image.
5.
Click the
Repeat
button on the Task List to mark the beginning of tasks that
will be repeated for every column.
6.
Add the
Wash Tip
command to the Task List by double-clicking the Wash Station
image.
Tip:
For additional help, see Installing LCP Attachments.
Repeat
