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NT8 User's Guide | Chapter 5: Hardware Overview
The NT8 deck is divided into the following stations:
1 – Waste Station.
The waste station is where the NT8 disposes of LV tips.
2 – Protein Source Station.
A protein sources station is a station for the
protein source that is included in the NT8 accessory kit.
Protein Source
The NT8 supports for low volume protein block and high volume
protein block. The protein source has three columns of eight drops
each (24 total), each well capable of holding 3 µL (LV protein block) or
13 µL (HV protein block); the protein source is required for the protein
source calibration. Use wells #1 through #8 at the first column (on the
left) of the protein source as the reference tuning points.
3 – Wash Station.
The Wash Station is a location on the NT8 for washing low volume
pipette tips.
4 – Low Volume (LV) Tip Caddy Station.
The low volume (LV) tip caddy station is a
repository that holds up to 384 replacement LV tips.
5, 6, 7 – Microplate Stations
. On the deck of the NT8 there are designated areas for three
SBS footprint microplates. Three microplates can be placed on the deck to transfer
reagents from one plate to another. A hanging drop cover sheet can be placed on station
#1 for setting hanging drops.
Reference Plate
Use the reference plate that is included in the NT8 accessory kit. The reference plate
has cross hairs in every corner for reference points (see the picture below, indicated by
orange circles). Use the top-left, top-right, and bottom-left cross hairs as the tuning
points.
8 – High Volume (HV) Tip Caddy Station.
The HV tip caddy station holds up to 96
replacement HV tips for the system.
9 – Deep Well Station.
The deep-well station is compatible with 96 deep-well plates,
which are used in daughter plates creation.
