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Follow the steps below to set up and run a Sitting Drop experiment. There are four overall
steps:
●
Set up the hardware
●
Set up the software
●
Add protein to the protein block
●
Run the task list you created
Set Up the NT8 Hardware
1.
Place the tip caddy and the MRC 2 Well Crystallization plate on the NT8. Each well
should contain 50 µL of solution.
2.
Place the protein tray on the protein block, and put the protein tray clamp on top of
the protein tray to hold it in place. You will pipette your protein into the protein
block after you set up the software.
Option 1: Set up the Software with the Sitting Drop Template
A Sitting Drop experiment setup template is pre-installed with your software. You may use it if
you plan to combine 100 nL well solution with 100 nL protein for a total dispense volume of
200 nL. You can also modify these volumes by double-clicking the item on the task list.
However, you must use the MRC 2 Well Crystallization plate.
