Fluorescence Assembly:
Page 17
Centering the Aperture Diaphragm:
Rotate the turntable to place the fluorescence filter B
or G into the light path. (If there is no fluorescence
filter B or G use another).
Rotate the 10x objective into the light path.
Put the shutter (1) to position “O” to open the light
path.
Focus on a slide on the stage.
Pull the aperture diaphragm lever (2) all the way out
to close the aperture diaphragm to its smallest
position.
Remove one eyepiece and replace it with the
centering telescope. Adjust the centering telescope
to find the image of the aperture diaphragm in the
field of view.
Adjust the two aperture diaphragm centering screws
(3) on the side of the illuminator with the inner hex
wrench to move the image into the center of the
field of view.
Open the aperture diaphragm gradually, if the image
is inside the field of view, the aperture diaphragm
has been centered properly.
In fluorescence observation, push the aperture
diaphragm lever (2) to open the aperture diaphragm
to the largest position.
The aperture diaphragm is centered at the factory
before shipment.
If a high-brightness excitation light is used, the
fluorescence of the sample will decrease. In this case
use the ND filter to reduce the brightness of the
excitation light. If no ND filter is available, narrow the
aperture diaphragm to obtain the same result.
