Step-by-Step Assembly:
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Centering the Condenser:
Rotate the condenser raise/lower knob (1) to raise
the condenser up to the highest position.
Rotate the spanner (2) on the condenser to move the
front lens into the light path. Use this condenser lens
in the light path when the objective is 20x or higher.
Move the 20x objective into the light path and focus
on the sample.
Rotate the field iris diaphragm adjustment ring (3) to
put the field diaphragm at the smallest position
where the field can be observed through the
eyepieces.
Rotate the condenser raise/lower knob to adjust the
image to its clearest.
Adjust the condenser centering screws (4) to place
the image in the center of the field of view.
Open the field iris diaphragm gradually. If the image
is in the center all the time and inscribed to the field
of view, the condenser has been centered properly.
(See field of view images at right).
When using the microscope you can enlarge the
field iris diaphragm a bit and make the image
circumscribed to the field of view.
Field Diaphragm Adjustment:
By limiting the diameter of light entering the
condenser, the field diaphragm can prevent other
light from entering the field of view and strengthen
the image contrast. When the image is just on the
edge of the field of view, the objective will perform
best and the clearest image will be obtained.
Rotate the field iris diaphragm adjustment ring (3)
clockwise to enlarge the field diaphragm. Rotate it
counterclockwise to close the field iris diaphragm.
Aperture Diaphragm Adjustment:
The aperture diaphragm determines the numerical
aperture (NA) of the illumination system. If the NA of
the illumination system matches the NA of the
objective lens, the best resolution, contrast and
depth of field is observed.
Adjust the aperture diaphragm ring (3) to control the
size of the diaphragm. If necessary remove the
eyepiece and observe from the eyetube while
adjusting the aperture diaphragm ring until the
scale of the condenser is set to 80% value of the
objective (4).
