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LS720 Microscope Manual 2021
7.
Click on the Image tab to select the channels to be used in
the Protocol and adjust their settings. If settings were
previously determined in Manual Image, transfer the
values into the current Protocol by clicking the Use
Manual Settings button. The desired values can also be
entered manually. Note that this dialog box cannot be
used to optimize the image settings because it is not
interactive with Live Image Window.
If optimization of any of the Channel settings has not been done or is still needed, click on the
Manual Image icon on the Left Toolbar (top icon) to open its dialog box and fine tune the settings
with a representative sample. When settings are satisfactory, click the Use Manual Settings
button in the Image tab to upload them into the Protocol.
8.
Click the fourth tab, Z-Stack, where you may enter the stack depth as well as the slice thickness.
This will cause an update to the number of slices in the stack. The stack will be centered on the
Autofocus result, the Z value of the Z-Map, or the Z-value of the ROI map. The center image will
always be captured in addition to the slices. If an even number of slices are requested, it will be in
addition to the slices and if an odd number are requested, the center position will be imaged twice.
The Z-Axis Starting position can be offset from the focus or Z value of the location.
9.
Click the fifth tab, X Y Z, to define the locations to be
imaged. Click Enable Stage to select location options.
10.
Click the Select Labware button to choose a labware type.
Locations can be selected by clicking on them individually or
clicking on the name of any row (A, B,
etc
) or column (1, 2,
etc
).
11.
Clicking
“
ROI positions chosen by user
”
will allow a worklist of positions referred to as an ROI Map to be
used for the Protocol. Defining this ROI Map is covered above.
12.
Images can be saved to individual folders for each location.
Check “Save to folders named for wells”.
If
you prefer images to go to one folder, uncheck the box. In Instrument Setup you may also choose the
setting that saves images into folders according to channel .
