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LS720 Microscope Manual 2021
2.
Information and values will be added on the various tabs. Be sure to click Save before leaving any
Protocol.
3.
In the first tab named Information, click the Load Existing button to bring
up a previously saved Protocol. If the desired Protocol is present, click OK.
Click the Clear Existing button if values have been entered on any of the
tabs and saving is not desired or a previous Protocol is displayed and you
want to start a new one. If unsaved entries are being cleared, a Save
Protocol File dialog box will open asking if you want to save the current
Protocol. If Yes, click OK.
4.
If not previously saved, enter the new desired Protocol name in the Filename field and click OK.
Or click on an existing File name to overwrite and a File Overwrite Alert will open; click Yes.
You are now ready to create a new Protocol.
5.
On the Information tab, if you enter User Notes, they will become part of the new Protocol. You
can also enter User Notes from a previously saved Protocol in this tab.
6.
Click on the Acquisition tab to select the acquisition mode:
Single Image, Time-Lapse, Video, or Time-Lapse Video.
a.
Single Image mode will snap an image of each location selected once and finish.
b.
For Time-Lapse, enter the Interval and Duration of your
time lapse experiment. Typical cancer cell lines use 10
minutes to 1 hour intervals for 24-72 hour duration. The
maximum Duration is 1000 hours or 43 days.
IMPORTANT: Before initiating a time-lapse run, it is critical to change your
computer power and Windows update settings so they cannot automatically
turn on and interfere with scheduled image captures. See Appendix D
c.
Live Video will record a video at each location using a single LED illumination
d.
Time-Lapse Video will take a short video at each location at the time lapse interval rather
