6
LS Microscope Startup Manual 2018.7.25
is directly connected to the LS Microscope, allowing it to be controlled by Lumaview. It should also be
used when imaging in a dark environment such as an incubator. To mount the Phase Accessory on an
LS Microscope, see Sections I.H. above. Brightfield images will be in gray scale due to the
monochromatic CMOS camera.
2.
If the Phase Contrast Accessory is not available, ambient light in a typically lit area can often be used.
The light should be as uniform as possible to prevent shadows or other dark or light areas. Overhead
fluorescent lighting can cause uneven brightness including a striped pattern on the image; if observed,
move the LS Microscope or partially shade the light to reduce unevenness across the sample.
J.
Installing Objectives
1.
LS720 only
a.
Unscrew the deck knob at the front of the instrument (side with LS720 logo)
and raise the top deck (the top layer of the hinged 2-layer deck). Tipping the
top deck backward will latch it into place. Unscrew the black cap (or
previously installed objective) on top of the optics block and screw in the
objective. Do not overtighten. To lower the top deck, activate the release
lever, lower the deck, and tighten the white deck knob.
2.
LS620, LS560, LS460 only
a.
Manually remove the insert of the top accessible deck. Unscrew the black cap (or previously
installed objective) on top of the inside optics block and screw in the new objective. Do not
overtighten. Return the removable insert to the deck.
3.
When installing or changing an objective, carry out the insertion quickly to minimize dust falling onto
the mirrored dichroic filter. A small amount of dust will not affect imaging. However if dust is
significant and it appears to affect quality of the live image or snapped images, contact Etaluma by
email (
) or telephone (760-298-2355) to discuss possible methods for cleaning.
IMPORTANT: Using compressed air can result in an oily liquid being sprayed onto the dichroic, which
may damage it. Similarly use of physical methods such as swabs or lens paper may also damage the
dichroic and must NOT be used.
K.
About Lumaview
1.
LS Microscopes LS720, LS620, LS600, LS560, and LS460 are controlled by the Lumaview software
program. The latest Lumaview version is downloadable from Etaluma’s website and
must be installed
prior to connecting your computer for the first time to the LS Microscope
(due to the need for two
drivers that are installed on the computer at the same time).
2.
Lumaview will run on Windows 7, 8, 8.1, and 10. Both desktop computers and laptops can be used,
but the best visualization correlates with monitor resolution equal to the sensor resolution (up to 1900
x 1900 pixels). Note: The monitor does not affect image resolution unless the monitor is low quality
and affects your ability to judge focus.
The computer should have Windows .NET Framework 4.5.2 or higher installed; see
https://msdn.microsoft.com/en-us/library/bb822049%28v=vs.110%29.aspx
for more information. If
Deck release lever
