LS Microscope Startup Manual 2018.7.25
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9.
On the right side of Instrument Setup, checking Display scale bar results in a scale bar in the lower right
corner of live and snapped images. If checked, click Select Color to choose color, check Display
objective power if desired, and select Scale bar width.
10.
After all settings have been selected, be sure to click OK to save the settings before exiting
Instrument Setup.
E. Finding a Live Image for Manual Focus
1.
Install the desired objective and sample labware.
LS620, LS560, and LS460 only
:
Manual XY movement of SBS labware (microplate footprint) is facilitated by
Etaluma’s Manual XY Stage (optional). For labware that is not SBS, use a labware
holder such as the Multi-Slide Holder for 1-4 microplate slides. Update Objective in
Instrument Setup as needed (Section II.D above) and click OK.
2.
Click on the Manual Image
icon in the Left Toolbar (top icon) to open its dialog box.
LS560
dialog box contains only the Green and Brightfield controls;
LS460
dialog box
contains only the Brightfield controls.
LS720 only
: Click the Manual XYZ icon in the
Left Toolbar (second icon from top) to open its dialog box; Manual Image and Manual
XYZ are used together to find and focus on samples in the labware. If desired, move
dialog boxes to the sides of the Live Image Window. If closed and then reopened,
they will open in the same locations prior to closing. However, when Lumaview is
closed, the dialog box positions will not be saved.
3.
LS720 only
: Select a desired XY location in a sample labware using one of two options
in Manual XYZ:
a.
Use the X/Y Move arrows (and Step size arrows if needed) to move to different
locations or wells in the sample labware, or
b.
Click on Select Labware to open its dialog box. Click on the labware type
desired to highlight it and click OK. The appropriate Labware Map will
then open. Click on a location and the microplate carrier will move so the
location is centered over the objective. For further movement from the
location, use the X/Y Move arrows (and Step size arrows if needed) in
Manual XYZ. Clicking again on the original location in Labware Map will re-
center on that location.
c.
Image orientation: The live image displayed on the monitor is as viewed
from the bottom as for all inverted microscopes. To see the live image as
viewed from the top, check Horz. Flip image in Manual XYZ. However, all
images are captured as viewed from the bottom.
d.
XY movement: In Manual XYZ, when a directional arrow in XY Move is clicked,
the live image on the monitor moves in that direction. However, the labware
moves in the opposite direction in order to place the new location over the
