LS Microscope Startup Manual 2018.7.25
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2.
LS620, LS560, LS460 only
a.
Unless approximate sample focus level is known, it is best to initially raise objective to maximum
height (without hitting sample) and then turn focus knob counter-clockwise until sample comes
into focus. Ensure you are focusing on correct sample plane and not another surface, for example,
back side of a microscope slide. If uncertain, place a similar but easy to focus sample on stage,
focus, and then switch to new sample.
b.
To facilitate the focusing process, click on the Zoom icon on the Left Tool Bar (fifth from top). The
Zoom feature toggles between displaying the entire image fitted to the Live Image Window and
displaying the image matched 1:1 with the monitor resolution (pixel: pixel). If the monitor
resolution in pixels is less than the frame size, the image will be enlarged (zoomed). The Live
Image Window will now show a center portion of the entire image. Click the Zoom icon again to fit
the image to the Live Image Window.
c.
If necessary, adjust Illumination and/or Gain as needed.
G.
Snapping Images
1.
When the desired field has been illuminated and focused in Live Image Window (Section II.F. above),
click the Camera icon in the Left Tool Bar (third from the top) to snap the image. If the Zoom function
was used to focus, the image captured will include the entire field of view (FOV) observable with the
Zoom off. If “Click” sound for manual image snap was checked in Instrument Setup (Section II.D.), a
shutter-like sound occurs when each image is snapped.
2.
If Manual image autosave was checked in Instrument Setup, images will be saved automatically using
the time-based numbering system (Section II.D.8.e.). If Manual image autosave was not checked, a
Save As dialog box will open after the image was snapped. Images will be saved in the Etaluma folder
formed automatically whenever Lumaview is downloaded and installed. See Appendix E for the
automatic subfolder directory that appears within the Etaluma folder.
3.
Images are captured and saved as seen by the camera, i.e., from the bottom.
LS720 only
: If desired,
images can be flipped horizontally to show how they would appear from the top. To accomplish this,
check “Horz flip image” in the Manual XYZ dialog box. The message “The image is flipped horizontally
and so appears as if viewed from above” is then displayed at the top of the dialog box.
4.
To view the last image snapped, click the Folder icon in the Left Tool Bar (bottom icon) to launch
Windows Explorer and open the most recently used Destination Folder.
H.
Auto-Generation of Single Composite Images
1.
Fluorescence
composites
(LS720 and LS620 only)
a.
In Instrument Setup (Section II.D.), make sure Auto Composite is
checked.
