LS Microscope Startup Manual 2018.7.25
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b.
Click the Composite icon in the Left Tool Bar (fourth from the top) to open its dialog box. Check the
channels desired. To use values optimized previously in Manual Image (Section II.E-F. above), click
Use Manual Settings to upload the settings. Values can also be entered manually.
c.
Click OK to start the snapping of individual images in progression. Each LED will turn on for the
pre-snap interval and then flash when the image is snapped. If “Click” sound for manual image
snap was checked in Instrument Setup, the shutter-like sound will be heard for each image.
d.
The fluorescence composite image will appear automatically in the Main Window matched 1:1
with the monitor resolution (pixel:pixel) and with its Title Bar showing the full path where it is
located.
2.
Composites including phase contrast or brightfield (
LS720, LS620, LS560)
a.
In Instrument Setup, make sure Auto Composite is checked.
b.
Configure a Protocol with the channels desired (See Section II.K. below). In the Acquisition tab,
select Time-Lapse and set both Interval and Duration to 1 sec. Click Run and after the images are
snapped, click the Last File icon in the Left Tool Bar (lowest icon). The folder containing the
individual images plus the Composite image will open.
I.
Live Video (Manual)
1.
When a desired field has been illuminated and focused in Live Image Window, click the Video Record
icon in the Left Tool Bar (fifth from the top) to open its dialog box. Select parameters requested and
also those in Instrument Setup such as file format (Section II.D.). Images captured will be saved to the
Video folder inside the Manual folder shown in Appendix E.
2.
Click OK and image capture will begin. To stop image capture, click the Record Vid
eo
icon in the Left
Tool Bar again.
J.
Videos from Time-Lapse and Live Video
Several options are available for compiling videos from sets of time-lapse (see Section II.L.) and live video
images:
1.
Lumaquant software offered by Etaluma (see Lumaquant Startup Guide for compiling images into
video). The Lumaquant Startup Guide can be downloaded from Etaluma’s website
(
www.etaluma.com/products/downloads/
).
2.
Other video compiling software such as ImageJ, Fiji, MSMoviemaker, iMovie.
3.
Two freeware software programs used together, i.e., AviSynth and MeGUI (instructions described in
Appendix F). If using this option, be sure to read the instructions first because the two programs must
be downloaded before image capture.
