ERBA ELite 5, User Manual

Page: 81 / 165

ELite 5, User Manual
Page 81 of 159
Warning flags
Warning flags are raised when the analyzer detects a problem in the analysis process that may cause
incorrect or suspect results or the parameters in the valid blank measurement was too high.
Flag Meaning Cause Action
l PLT URI
The PLT-RBC gap is not clearly detectable on the
PLT-RBC histogram: small MCV, fractured RBC-s,
aggregated PLT-s (cold blood), side-effect of blood-
transfusion.
Check the sample quality. Repeat the sample. If the
problem persists then perform a manual count on a
stained smear.
M
Out of RBC linearity
range
The RBC coincidence is over the limit: too high RBC
count.
Check the sample homogeneity. Repeat the sample
with manual pre-dilution.
m
Close to RBC linearity
range
The RBC coincidence is close to the limit: too high
RBC count.
Treat RBC result as ‘reduced reliability’. Check the
sample homogeneity. Repeat the sample with manual
pre-dilution.
N High angle noise
The flow in the optical head is not clean or contains
bubbles Perform a blank measurement. If noise still appears,
make a flow cell backwash.
n Low angle noise
The flow in the optical head is not clean or contains
bubbles Perform a blank measurement. If noise still appears,
make a flow cell backwash.
O
High linearity range
limit exceeded If any of RBC,PLT or WBC results are higher than
high linearity range limits. Perform manual pre-dilution of sample and re-
measure it.
o
Low linearity range
limit exceeded If any of RBC,PLT or WBC results are lower than Low
linearity range limits. None
p PLT blank high Last accepted blank result: PLT
≥
15 * 103 cells/µl
Check cleanliness of the reagents and the ‘ELite 5’.
Perform cleaning of the ’ELite 5’. Repeat blank
measurement. See chapter: 6.2.5:Pneumatics start
and blank measurement
Treat PLT result as ‘reduced reliability’.
Q MON - LYM alert
The MON and LYM populations are not clearly
distinguishable on the scatter-gram.
If other parameters predict a ‘normal’ sample then
perform a cleaning procedure and repeat the
measurement.
If MON, MON%, LYM, LYM% results reported then
treat them as ‘reduced reliability’. If not all the
parameters reported or the sample is predicted as
pathological then perform a manual count on a stained
smear.