Biometra TAdvanced
Creating, editing and saving programs
Version 02_20
53
G
RADIENT
There are two options to program a gradient. To toggle between the two options,
press the corresponding button on the Biometra TAdvanced gradient programming
screen (see Fig. 31):
L
INEAR
G
RADIENT
T
OOL
S
TANDARD
G
RADIENT
P
ROGRAMMING
To program a gradient using the Linear Gradient Tool, enter the desired annealing
temperature (A
NNEALING
T
EMP
.) and define a temperature increment (G
RAD INCR
.)
between the individual columns of the sample block. The effective temperatures for all
the other columns are automatically calculated and displayed as a bar chart (see Fig.
31).
Note
: The annealing temperature is set for a specific column in the sample block. For
example, the annealing temperature in a 96 well sample block corresponds to the
temperature in column 6. The column number is indicated in parenthesis next to the
corresponding input field (see Fig. 31).
When setting a positive increment, the temperature in column 1 is the lowest and the
temperature in column 12 is the highest. Use the minus button to enter a negative
increment. When a negative value is used, the temperature in column 1 is the highest
and the temperature in column 12 is the lowest.
Fig. 31 Linear Gradient Tool screen (Biometra TAdvanced Mono)
To create a gradient using the standard gradient programming screen, enter a
temperature for the first column and the last column in the sample block (see Fig. 32).
The effective temperatures for all the other columns are automatically calculated and
displayed as a bar chart.