Creating, editing and saving programs
Biometra TAdvanced
50
Version 02_20
Fig. 28 Program step editing screen
Note
: To program a step at least the temperature and the hold time must be defined.
3.
To set the temperature, touch the corresponding input field and enter a value
between 3.0°C and 99.0°C. Use the [
TAB
] button or activate the next input field and
enter a hold time in
H
:
MM
:
SS
.
The Biometra TAdvanced software allows several program steps to be programmed
subsequently (Multi Step Programming).
4.
Use the cursor keys to navigate between program steps.
N
EXT STEP
P
REVIOUS STEP
The currently activated step is highlighted in blue in the table above the input fields
(see Fig. 28 on page 48).
Note
: By navigating between program steps it is not necessary to confirm the settings
for each single parameter or step and to leave the screen for editing program steps.
Instead, you can fill in the parameters for all steps subsequently and confirm your
entries after all steps are programmed.
A typical PCR program consists of repetitive steps for denaturation, annealing and
extension. For the repetition of steps the Biometra TAdvanced software allows the
programming of loops. For a loop two parameters have to be set:
The step number to which the program should return (G
O
T
O
)
The number of repetitions (C
YCLES
)
1.
To program a loop, select the last step of the loop and enter the step number to
which the program should return in the G
O
T
O
input field (see Fig. 28).
2.
To define the number of repetitions, enter a number in the C
YCLES
field
(see Fig. 28).
For example, to program the loop for the following protocol, select step 4 and enter
the value 2 in the G
O
T
O
input field and the value 35 in the C
YCLES
input field.
Programming loops