Creating, editing and saving programs
Biometra TAdvanced
52
Version 02_20
The average ramp rate can be adjusted for each step. This adjustment can be useful if
PCR programs are transferred from slower devices to the Biometra TAdvanced or for
special PCR applications, for example, telomerase PCR. The average ramp rate
specifies the speed at which the selected step is reached with.
To adjust the ramp, press the following button (see Fig. 28):
R
AMP RATE
(
R)
Then enter a value between 0.1°C/s and max. in tenths of degree Celsius.
It is also possible that the entered ramp rate is applied to all steps in the PCR
program. For this the following checkbox to the right of the input field for the ramp
rate must be activated.
Note
: The maximum average ramp rate depends on the sample block installed in the
Biometra TAdvanced. If the ramp rate has been adjusted, the small symbol
will be
shown at the corresponding step in the graphical programming mode.
For Biometra TAdvanced monoblock models
Note
: To be able to use the gradient function, the sample block needs to be gradient
enabled.
The gradient function is most often used to optimize new primer pairs. A temperature
gradient is established across the sample block in the annealing step so that there are
different temperatures from column to column:
Fig. 30 Temperature gradient on a 96 well sample block
In a specific column all wells have the same temperature but the temperature differs
from column to column. The temperature gradient can be applied in both directions;
the highest temperature can be either in column 1 or column 12.
Sample replicates are distributed across the columns of the sample block and tested for
the temperature giving the optimal result. To program a gradient step press the
following key (see Fig. 28)
Adjusting the ramp rate
Programming a gradient
step