Contents
1.
Important Checklist Before Operation ............................................. 1
2.
Terminology .......................................................................................... 2
3.
A List of Fitting Parts and Directions for Use ...................................... 3
4.
Mobile Phase Preparation.................................................................. 4
5.
System Installation ............................................................................... 5
5-1.
Wiring .......................................................................................... 5
5-2.
Tubing and Degasser ............................................................... 5
5-3.
Connecting to the Autoinjector and the Autosampler
(Plumbing) .........................................................................................
5
5-4.
Connecting to the Autoinjector and the Autosampler
(Electrical Signal Connection) .......................................................
6
6.
Online Elution Degassing System ...................................................... 7
6-1.
If you think the degassing efficiency is reduced,
you may diagnose the problem by the
following procedure: .................................................... 7
7.
HPLC Pump Operation ....................................................................... 8
7-1.
Run/Stop .................................................................................... 9
7-2.
Flow Rate Setting ...................................................................... 9
7-3.
Pulse Free Mode Setting .......................................................... 10
7-4.
Function of Pulse Free Mode .................................................. 10
8.
How to Fix Pump Errors and Problems .............................................. 11
8-1.
Less Flow Rate, Less Pump Pressure or
Unstable Pump Pressure ............................................... 11
8-2.
Types of Pump Errors ................................................................ 12
8-3.
How to Fix these Errors .............................................................. 13
