1
HTEC-500
1.
Important Checklist Before Operation
• The separation column and the enzyme column must be held firmly by the column clip. The stainless
steel surfaces should make good contact with each other in order to ground static electricity. This
may be the cause of noise in the ECD.
• Place the mobile phase container and the waste container on the same level as the HTEC-500.
• Use glass bottles as mobile phase and ultrapure water containers. Do not use any plastic containers.
• Rinse behind the pump pistons with ultrapure water before and after use through the pump piston
washing port. Do not use any organic solvents in the rinse solution. If precipitation or something blocks
the line, do not push out with high pressure or force. First, disconnect the line and then remove the
source of the blocking.
• Before opening the detector cell for maintenance, remove the outlet screw and aspirate all the
remaining mobile phase in the detector cell with a syringe.
• Pay attention to prevent any tubing from becoming blocked. Salt solvents must be rinsed out by
ultrapure water before an organic solvent of a high concentration is used. Drying out of the salt
solvent should also be avoided.
• Pay attention not to allow any leakage from any connection points of the tubing. Leakage is not only
harmful for the equipment but also generates pump pulse noise.
• When air is thought to be present in the tubing or the pump, please purge the air (see chapter 8,
page 11). Then you can connect a column.
• Pulse free mode must be switched on during the analysis.
Column Clip
Separation Column (PP-ODS)
Door
A separation column must be held firmly by the column clip.
When an enzyme column is used, the enzyme column must
also be held firmly by the column clip. The respective stainless
steel surfaces should make good contact in order to ground
static electricity. This can be the cause of noise in the ECD.
Fig. 1 Separation Column Installation
