20
HTEC-500
10-3. How to Repack
Disconnect the precolumn as shown below and
take the packing material away. Then wash the
inside of the precolumn with running water and
then rinse it with ultrapure water. For every two
times you repack, please exchange the upstream
filter once as described in chapter 10-4, page 21.
Put the precolumn packing material in methanol (it will become like a slurry type solution). Add
methanol to the bottle containing the packing material, put the cap on and shake well. Connect a
syringe union to a disposable syringe filled with 0.5 ml methanol as shown below. Fill up the precolumn
with the packing material in the methanol using a pipette. Then slowly suck methanol out of the
precolumn using the disposable syringe.
Repeat these steps until the precolumn is full of the
packing material (the packing material must rise up
from the inside of the precolumn). Then tighten the
two parts using a wrench.
Important
Aspirate the methanol using the syringe slowly.
Fill up the precolumn with the packing material
before the inside of the precolumn becomes
dry. Avoid any gap in the precolumn.
Fill up the syringe with fresh methanol after
disposing of the methanol collected during the
packing procedure. Connect the syringe to the
inlet of the precolumn and wash the precolumn
using this syringe (see Fig. 19). Repeat this step
twice. Then wash the packed precolumn with
ultrapure water in the same way as described
above.
After these steps, connect the precolumn to the line again. First, connect the tube of the upstream side
only and wash inside by pumping mobile phase through. After several minutes, connect the tube of the
downstream side. Except in the case where the precolumn is located before the injector, if the
repacking of the precolumn was not successful, the shapes of the peaks will be abnormal.
If the shape of the peak of a standard sample with the repacked precolumn doesn’t become better
and the shape of the peak is good without a precolumn, the repacked precolumn is not good. Try to
repack the precolumn again. If the specified packing material was not used for repacking, then the
shape of the peak will be asymmetric.
Upstream
Flow
Fig. 17
Fig. 18
Fig. 19
