8
HTEC-500
7.
HPLC Pump Operation
Pay attention to the following points about the pump operation:
• Rinse behind the pump pistons with ultrapure water before and after use. Do not use any organic
solvents in the rinse solution. It is common that an air bubble or air segment may appear in the piston
wash line but it does not affect any functions.
• The mobile phase container should be a clean glass bottle. Do not use a plastic container.
• Put the mobile phase container on the same level as the HTEC-500.
Pump Outlet
Piston Wash Port
Check Valve (Outlet)
Pump Head
Piston Wash Port
Check Valve (Inlet)
nut
PEEK Tubing 0.75 mm ID
Easy Fit
(Tubing connected to the piston wash ports is silicon 3 1 mm)
PEEK Tubing 0.5 mm ID
OPEN
Drain
Fig. 7 Pump Parts and Drain
The drain valve and the drain port are integrated. Connect a syringe
to the drain port (as in the figure above) and turn the drain valve a
half circle. Use the syringe to suck out some mobile phase.
Check Valve
(Outlet)
Check Valve (Inlet)
Pump Head
Piston Seal
Backup Ring
O ring
Wash Port
Head Guide
Piston
Diaphagm
Center Guide
Rod Assay
Fig. 8 Pump Details
