16
HTEC-500
9.
Manual Sample Injector and Autoinjector
9-1.
Structure
At the back of the manual injector, the sample loop is connected to ports No. 1 and No. 4. The tubing
from the carrier pumps is joined to no. 2 port. No. 3 port outputs to the column. No. 5 and 6 are drain
ports. Whenever injecting into the manual injector, use the accessory syringe. If you need to inject more
than 25
μ
l, please order the 50
μ
l syringe (Eicom model number 705SNR). Do not connect any other
tubings to the drain port. This is to avoid any potential problems.
9-2.
Operation of Manual Injector
Caution!
Do not use a syringe with a sharp needle. Always use a blunt needle.
The manual sample injector switches the flow by changing the lever knob position from LOAD to INJECT.
When introducing the sample into the manual sample injector, only the Hamilton Microsyringe type
702SNR or 705SNR is recommended.
A. Aspirate the sample into the microsyringe. If an air bubble generates inside of the syringe, please
flush out the solution with the air bubble and slowly re-aspirate. Generally the knob is set at INJECT
before and after the injection.
B. Insert the syringe needle until you feel a strong resistance (3 mm further than the point where you
feel a weak resistance).
C. Turn the knob to LOAD and push the plunger to inject the sample into the manual injector. Through
this step, the sample is injected into the loop.
D. Turn the knob from LOAD to INJECT immediately and smoothly. By moving the knob from LOAD to
INJECT, an electrical signal is also generated.
E. After completing these steps, remove the syringe and wash the syringe with ultrapure water. Please
wash the entire syringe each time between injections to avoid contamination.
After each injection, the needle port of the manual injector must be washed with ultrapure water
using the white port cleaner and a syringe.
When using an external injector, please refer to it’s user’s manual.
When comparing the concentration of different samples, inject the same volume every time.
Wash the syringe with hydrochloride periodically to keep it clean.
