DORIC BFP Quick Starting Manual Download Page 13

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BFP SYSTEM :

Quick Starting Guide

4.6.3. Time Series :

Time Series allow to perform long term recording with long delay between recording sessions; i.e. 1
minute of recording every hour for 12 hours, as per example above. This will create a single file with all
the datas, NOT a file for each serie.

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Summary of Contents for BFP

Page 1: ...FIBER PHOTOMETRY BFP SYSTEM QUICK STARTING GUIDE DORIC LENSES INC 357 rue Franquet Qu bec Qu bec G1P 4N7 CANADA Tel 418 877 5600 Fax 418 877 1008 www doriclenses com Date 2020 02 28 Rev 00 Copyright...

Page 2: ...excitations ports for light coming from LED light sources The LED source current is adjusted by the LED driver LEDD The BFP Driver BFPD is the key component to synchronize the LED driver with camera a...

Page 3: ...with the system Launch Cam33U_setup_4 7 0 1557 exe 2 2 Install Doric Neuroscience Studio using the version on the USB key supplied with the system Launch setup_DoricStudio5 4 0 15 exe Or looks for th...

Page 4: ...irrors and bandpass spectral filters with optics to have uniform illumination on optical fibre port that is re imaged to an image snesor In most common configuration the excitation LED are integrated...

Page 5: ...C 1 2 3 BNC 3 4 USB hub Use the jack Y splitter to power the LED driver and the USB hub Connect the USB3 cables between cameras and the USB hub port 1 and 2 Connect the USB2 cable between the LED driv...

Page 6: ...ect the TTL mode and set the LED current using the software by clicking Add Channel in the Configuration Tab as per figure below In order to set the excitation power you need to monitor the optical po...

Page 7: ...r mode There is a minimum current threshold of 40 mA in normal mode and 4 mA in low power mode Below this value LED will turn OFF completely 4 3 BFP Set New Configuration Configuration Tab In the BFP...

Page 8: ...rd excitation 3 only i e RcaMP shorter exposure time but NO crosstalk between excitations 4 1 1 1 Excitation LED 1 continuous Camera 1 record excitation 1 to record faster event without interleaving O...

Page 9: ...B Trigger Mode Gated You should click LIVE or RECORD in the Acquisition Tab to have device ready to record and recording will start when the selected DIO receive TTL high signal 5V Recording will con...

Page 10: ...for each optical fibre plus one outside to monitor the background A graph of the ROI average count vs time will appear and a row in right table for each ROI You can rename each ROI by doubleclicking o...

Page 11: ...nal you can increase the gain it will also amplify electronic noise then reducing SNR but it is a good way to increase sensitivity In our software we use a resolution lower than maximum camera resolut...

Page 12: ...Saving Options By default we record the average values of all ROI Digital I O if any have been set and the key event that create a timestamp everytime user presses the F1 F2 F3 or F4 key In some compu...

Page 13: ...rform long term recording with long delay between recording sessions i e 1 minute of recording every hour for 12 hours as per example above This will create a single file with all the datas NOT a file...

Page 14: ...fill available space in your screen If you prefer another zoom factor then uncheck the Fit in screen box and choose the desired Zoom factor When a zoom factor that requires to drag the display to see...

Page 15: ...ge acquisition to adjust focus Loosen the counter nut and rotate the fiber adpater until you get a clear image of the fiber bundle image in focus Be careful you may need to rotate several turns to adj...

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