CI-340 Operation Manual
CID Bio-Science
1554 NE 3
rd
Ave
Camas, WA 98607, USA
Phone: +1 (360) 833-8835
Fax: +1 (360) 833-1914
[email protected]
www.cid-inc.com
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Photosynthesis, Transpiration and Stomatal Conductance
Photosynthesis is the formation of carbohydrates from CO
2
and a source of hydrogen (as water) in the
chlorophyll-containing tissues of plants exposed to light. The rate at which photosynthesis occurs is
determined by measuring the rate at which a known leaf area assimilates the CO
2
concentration in a
given time.
It is known that transpiration is the primary determinant of leaf energy balance and plant water status.
The rate of transpiration is determined by the accumulation of water vapor flux per one-sided leaf area
in a given time.
Stomatal conductance is the water loss of a leaf. Conductance can be considered in parallel or series. It
can be obtained by measuring the transpiration and leaf surface temperature (°C), and applying the
calculation for Equation 4 found in the EQUATIONS chapter.
The CI-340 is designed so that measurements can incorporate both absolute and differential readings
simultaneously. This instrument can also be configured to operate both open and closed systems. It is
important that the CI-
340 uses the manufacturer’s current software.
Taking Measurements
Now, with all the necessary setup/calibrations completed, the CI-340 is ready to begin its
measurements. With any experiment, coding or naming a set of data points/values would help
characterize that particular group for computational requirements. This instrument is designed to do
such by allowing for a file name and time intervals prior to any measurements or data collected.
With the system powered on and at rest, press the
key. The instrument will display:
File name: ?
This filename would be similar to a DOS-format and limited to 12 characters (including the decimal
point). For example, to save a data set as “group 1”, the filename “GROUP1” could be used. Use any
recognizable
filename including any necessary extension (i.e., “GROUP1.C50”) format other than using
DOS executable extension (such as .EXE, .BAT, .COM, etc.). Note that pressing the
and
keys
will not ask for a file name to be entered, and measured data will not be saved.
Summary of Contents for CI-340
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