Doc. No.: 994-2029 v. 1.1 · Issue date: 03-Apr-2020
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ChemoMetec A/S · Gydevang 43 · 3450 Allerod · Denmark · [email protected] · www.chemometec.com
Figure 2. NucleoCounter
®
NC-202™ viability correlates with manual assessment by trypan blue. The graph presents
data from 15 different cell types with 166 measurements using three NucleoCounter
®
NC-202™ instruments and
manual counting.
Cell counting precision
The precision of a cell count depends on the number of cells counted. The variation of a cell count is assumed
to follow the Poisson probability distribution, where measurements of discrete events will deviate with the
square root of the number of events counted. In addition, variation in sample collection and processing will
also contribute to the overall deviation.
To demonstrate counting precision, the coefficient of variation (CV) was calculated from replica
NucleoCounter®
NC-202
™
or manual counts and plotted against the cell concentration (Figure 3). The
NucleoCounter NC-202
™
showed significantly lower variation; on average 4.1% (Figure 3A) as compared to
an average of 8.2% for manual counting (Figure 3B).
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-202™
Manual counting
Viability (%)
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Summary of Contents for NucleoCounter NC-202
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