24
Please note that the instrument lid height must not be adjusted at any point during calibration.
If the lid height is adjusted, a re-calibration is required.
PCR tubes used for the calibration process must be the same tubes that will be used for
subsequent test runs.
FAM + HEX Calibrator Set
Table 3.11
FAM + HEX
Calibrator
Set
FAM Calibrator
HEX
Calibrator
Reconstitution
Buffer
# of
Calibrations
Catalog
#
Shipped with
instrument
1x 0.66 nmol/vial
1x 1.32
nmol/vial
2x 1.34 mL/vial
3
-
Available for
order
2x 0.66 nmol/vial
2x 1.32
nmol/vial
1x 5.5 mL/bottle
6
S0140S
The final concentrations of the reconstituted FAM and HEX calibrators are at 500 nM and
1000 nM, respectively.
Storage Temperature
Store the lyophilized calibrators and the reconstitution buffer between 4 – 20
°
C. The calibrators
are light sensitive. Ensure that the vials are placed in a dark location. Storage temperature of
the reconstituted calibrators should be at 4
°
C and protected from light.
When stored at the recommended temperature and condition, the lyophilized calibrator and
reconstitution buffer are stable for two years from the date of manufacture. The reconstituted
calibrator is stable for one year when stored at 4
°
C and protected from light.
Do not freeze the calibrator once reconstituted.
Materials Required for Calibration
o
FAM/HEX calibrator solution
o
Distilled or deionized water
o
200
µ
L pipette
o
200
µ
L pipette filter tips
o
Chai PCR Tube & Cap Strips, 8-Well Strips, Optically Clear, 100
µ
L
o
PCR tube rack
