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: Returns to the home screen
Experimental Properties
: Displays the lid temperature throughout the experiment
View Protocol
: Displays the initial protocol setup page for the experiment
View Result
: Displays the results screen
Export
: Downloads the experiment file for further data analysis.
Delete
: Permanently deletes the experiment run from the database
5.5 Data Export
Data can be exported via a .csv file. To export the run file, click on the
icon at the top left of
the page. You will then see the following window on the left. Select
Export
to download the run
file.
Figure 5.14
Amplification runs contain the following files: amplification, cq, and temperature_log.
To determine the absolute copy number from the starting material, use the cq.csv file to
generate the standard curve.
o
amplification.csv
contains the baseline subtracted, background subtracted, and sample
fluorescence values for each corresponding channel, well, and cycle number.
o
cq.csv
provides the Cq values for each channel and well in the instrument.
o
temperature_log.csv
contains data for the lid and heat block temperatures per cycling
period.
Melt curve analysis contains the following files: melt_curve_analysis, melt_curve_data, and
temperature_log
o
melt_curve_data.csv
contains the normalized and derivative values for each
incremental temperature point
o
melt_curve_analysis.csv
contains the Tm(s) for each sample
