34
Figure 4.10
DEFINE CYCLE NUMBER
To set the number of cycles for a certain stage, click directly on the underlined default
40x
Cycles
shown in Figure 4.11 and type in the value.
Figure 4.11
EDIT STAGES
To delete or reposition a step, click on
Edit Stages
located on the right-hand side. To delete a
step, select the
Ä
icon. To reposition a step in the protocol, click and drag the dotted icon at the
bottom of the orange section.
Figure 4.12
ENDPOINT REACTIONS
For end-point reactions with no fluorescence detection (conventional PCR), make sure to turn
off
Gather Data
for Step and/or Ramp in all stages of your protocol. No amplification curves will
be displayed on the results screen with endpoint reaction settings.
Figure 4.13
