20
Preparing for Calibration
The Fluorescein solution is light sensitive. Keep aliquoted solution protected from light.
If you are using calibrators that have previously been stored at 4
°
C, you do not need to
equilibrate the solution to room temperature before running the calibration process. You
may use the calibrator solution directly from the fridge.
1.
Obtain 4 new 8-well 100
µ
L PCR tube strips. Check that there are no cracks on the
tubes.
2.
Vortex for 10 seconds or invert the fluorescein vial 10 times to ensure homogenous
mixing of the solution.
3.
Spin down the vial before aliquoting 25
µ
L/tube of the solution into 2 new 8-well PCR
tube strips. Cap tubes tightly and spin down.
4.
Prepare the water strips by aliquoting 25
µ
L/tube of water into 2 new PCR strip tubes.
Cap strip tubes tightly and spin down.
Calibration Procedures
Please note that the calibration process must be completed in one sitting.
The heat block and lid temperature rises above 95
°
C. To avoid injury, do not make
direct contact with the heat block, lid, or tubes immediately after a run. Let the surface
cool for a few minutes before removing the tubes. If samples must be removed
immediately, avoid contact with surfaces by extracting tubes with tweezers.
Always wear gloves and protective eye wear when handling reagents or operating the
instrument.
1.
Log into your account through a web browser. On the software home screen, navigate to
Settings
→
Calibration
.
2.
Under Optical Calibration, select
Run Now
.
3.
The calibration time and materials required will be shown on the next screen (Figure
3.3). Select
Begin.
