Cellink BIO X 3D Bioprinter User Manual Download | Manualshive

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Printheads pressure control

This allows you to test the pressure needed for the printhead to extrude correctly the

material being used. This pressure mainly depends on the viscosity of the material.

4.4 Utilities

2

1

1.

Set pressure for each printhead (0-200kPa, when using the internal air

compressor).

2.

Test bioink flow - Press the droplet button to assess the bioink flow.

Each droplet button correspond to one printhead for each print head.

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Summary of Contents for BIO X 3D Bioprinter

Page 1: ...User Manual BIO X 3D Bioprinter...

Page 2: ...ivalled usability and versatility BIO X will help in taking your research to the next level With features such as interchangeable intelligent printheads clean chambertechnology printchamber sterilizat...

Page 3: ...X 16 3 2 Contents of the box 17 3 3 Setting up BIO X 19 3 4 Starting BIO X for the first time 20 4 Bioprinting with BIO X 22 4 1 Sterilizing using UV light 23 4 2 Bioprinting from a 3D model 24 4 3 B...

Page 4: ...ISO 9001...

Page 5: ......

Page 6: ...and the CELLINK intelligent printheads are only to be used with each other not with other equipment This symbol indicates that there is risk of personal injury or equipment damage due to harmful UV ra...

Page 7: ...is a heavy instrument Always have a minimum of two people when unpacking or moving BIO X Always lift and carry the instrument in the manner described in the manual Please read through the instruction...

Page 8: ...for any warranty condition representation or term the extent to which cannot be excluded or limited by applicable law CELLINK and its suppliers make no warranty condition representation or term expres...

Page 9: ...9...

Page 10: ...Specifications...

Page 11: ...2b 1 Clean Chamber tm unit 2a UV sterilization LED 1 2b UV sterilization LED 2 3a Printhead mount 1 3b Printhead mount 2 3c Printhead mount 3 4a Photo curing module mount 1 4b Photo curing module moun...

Page 12: ...12 2 1 Product map 5 6 7 2 1a 1b 3 4 1a 1b 2 3 4 5 6 7 Heat vent from the printbed Heat vent from the printbed Power switch Fuse Power socket USB port Ethernet port Pneumatic port...

Page 13: ...13 2 2 Technical specifications Power input 100 240 VAC 50 60 Hz 600 W Fuse 250V T8A Air pressure input 0 700 kPa Weight 17 kg Height 500 mm Width 360 mm Depth 450 mm Filter class HEPA 14...

Page 14: ...14...

Page 15: ...Getting started...

Page 16: ...y table and a floor space of at least 3 x 2 meters 2 Place the box containing BIO X on its side in accordance to the arrows on the side of the box 3 Carefully remove the tape on the top of the box 4 U...

Page 17: ...sheet and Application note 5x 3mL CELLINK GelMa bioink sterile 1x Material safety data sheet and Application note 2x 3mL CELLINK START bioink sterile 1x Material safety data sheet and Application not...

Page 18: ...recision conical nozzles 50 pcs Blue GA 22 1x Sterile high precision conical nozzles 50 pcs Red GA 25 1x Sterile high precision conical nozzles 50 pcs Clear GA 27 1x Sterile blunt needles 20G 50 pcs 1...

Page 19: ...eads If you wish to use a higher pressure than this connect an external air supply to the back of BIO X see 2 1 Product map Next switch on the instrument using the power switch on the back see 2 1 Pro...

Page 20: ...ar Symbols in this area will alert you of potential hazards such as hot surfaces or active UV lights see the symbols below Use caution when these symbols are displayed 3 Bioprint Tap to start a 3D Bio...

Page 21: ......

Page 22: ...Bioprinting...

Page 23: ...vironment BIO X is equipped with a UV sterilization system which will create a sterile printchamber which is then maintained sterile by the Clean Chamber Technology CELLINK recommends running the UV s...

Page 24: ...remain connected to BIO X until the bioprinting process has begun Note The USB Drive needs to be read and writeable Next choose the model you wish to bioprint BIO X accepts the file formats stl obj a...

Page 25: ...at you want to print on and the size of the surface 1 2 3 1 Petri dish Select to bioprint on a petri dish This is a versatile option useful for many different applications 2 Well plate Select to biopr...

Page 26: ...to toggle between the settings for the different printheads and the printbed 2 Toggle printhead Tap to select if you want to use the printhead or not If you select to use the printhead further settin...

Page 27: ...printhead parameters change depending on what type of printhead you decide to use 1 Nozzle type Tap to select what type and size of nozzle you want to use 2 Printhead settings These settings change d...

Page 28: ...ed You can choose between having the printbed at a certain temperature or to turn the temperature control off and bioprint at the ambient temperature 1 Clean chamber fan Toggle the clean chamber fan o...

Page 29: ...sen layer profile can be seen here 2 Preview slider Drag this to select which layer to preview 3 Infill pattern Tap to choose infill pattern 4 Printhead use Tap to select which part of the model each...

Page 30: ...nd compression Linear pattern The linear pattern is suitable as an infill for tissue constructs that must process anisotropic mechanical characteristics Tissues such as ligament tendon muscle and nerv...

Page 31: ...rint a support structure for your 3D model Use this option if you are planning to bioprint a structure which cannot support itself 4 2 Bioprinting from a 3D model Layer settings Printhead use In the P...

Page 32: ...incorrect you can easily go back to that step and correct it by pressing the navigation bar at the bottom of the screen 1 4 2 5 3 6 1 Printhead 1 Settings for printhead 1 2 Printhead 2 Settings for pr...

Page 33: ...d print surface in BIO X By following your settings on the screen it is easy to set up BIO X correctly for your bioprinting process Remember not to start the bioprinting process before you have correc...

Page 34: ...will start When inserting your print surface use the spring loaded arms to securely fasten it according to the figure below If you are using a well plate simply turn the arms to the sides Note If you...

Page 35: ...printhead 3 4 Surface probe calibration tab for the surface probe 5 Enable ABL Toggle the ABL option on off The ABL should be enabled after calibrating the surface probe and going back to the Printhea...

Page 36: ...t UV curing module Allows you to monitor the specific properties of the left UV curing module 5 Right UV curing module Allows you to monitor the specific properties of the right UV curing module 6 Z O...

Page 37: ...u can choose to cancel the printing or resume 7 8 9 7 Pause Tap to pause the bioprinting process 8 Resume Tap to resume the bioprinting process This option is only available when the bioprinting proce...

Page 38: ...ption to start bioprinting droplet without having to create a gcode for it BIO X will guide you through the process and help you to set up for a perfect bioprint Choose Print surface Select the wellpl...

Page 39: ...o use the printhead or not If you select to use the printhead further settings will appear 3 Bioink profile Select the bioink profile you want to use or create a new one BIO X comes preloaded with pro...

Page 40: ...he settings can be reviewed Should a setting or value be incorrect you can easily go back to that step and correct it by pressing the navigation bar at the bottom of the screen 1 1 Printhead 1 Setting...

Page 41: ...oggle the ABL option on off The ABL should be enabled after calibrating the surface probe and going back to the Printhead 1 tab 6 Z axis command controls the position of the printbed along the z axis...

Page 42: ...erview of how far the bioprinting process has come and how long it will take until it is completed 3 Bioprint Tap to start the bioprinting process Caution Do not open the door without pausing the biop...

Page 43: ...e USB drive insert it in the Bio X You can then access it via this tab and procede to the updating of your Bio X 4 4 Utilities 2 1 1 USB content list allows you to select the desired update in the lis...

Page 44: ...want to start Bioprinting You can also choose to pre cool the printbed 4 4 Utilities 2 4 3 1 Printhead 1 temperature Use this to set the temperature of the printhead 1 2 Printhead 2 temperature Use t...

Page 45: ...aterial being used This pressure mainly depends on the viscosity of the material 4 4 Utilities 2 1 1 Set pressure for each printhead 0 200kPa when using the internal air compressor 2 Test bioink flow...

Page 46: ...stomer support purposes 4 4 Utilities 1 2 3 1 Enable dark mode turn off all the chamber lights This allows you to work with photo sensitive material 2 Clean chamber fan Toggle the clean chamber fan op...

Page 47: ...5 6 1 2 3 1 Printhead 1 test movement for printhead 1 2 Printhead 2 test movement for printhead 2 3 Printhead 3 test movement for printhead 3 4 Z axis command controls the position of the printbed alo...

Page 48: ...us to check and test the different sensors 4 4 Utilities 1 2 3 2 3 1 Output data Allows customer support to check and test the different censors 2 License Gives you access to all the licenses of the...

Page 49: ...Maintenance...

Page 50: ...ves and sterilize them using 70 ethanol Remove the four screws holding the HEPA filter in place 2 Replace the HEPA filter with a clean one Fasten the clean HEPA filter with the four screws and plug in...

Page 51: ...ug BIO X before cleaning it with ethanol Then simply spray the surface you wish to sterilize and wipe the surface with a soft cloth Caution If ethanol has been used to clean the printchamber of BIO X...

Page 52: ......

Page 53: ...Terms and Conditions...

Page 54: ...o assume that a delay in the agreed delivery could occur Cellink shall notify the Buyer thereof and shall in such case be entitled to reasonable prolongation of the delivery date of up to twenty 20 bu...

Page 55: ...any partial delivery will be regarded as filling a specific order 5 ACCEPTANCE The Buyer shall immediately following each delivery of any Product inspect the Product in order to identify any external...

Page 56: ...writing within the above time limits the Buyer forfeits its right to make any claim in respect of the defect If there is reason to believe that the defect may cause damage notice shall be given forthw...

Page 57: ...s only allowed to use the Product Software for your own benefit and you are not allowed to make the application available to third parties 9 TRADEMARKS AND OTHER INTELLECTUAL PROPERTY RIGHTS All intel...

Page 58: ...event that a Product or parts thereof becomes subject to a recall decided by Cellink the Buyer shall participate and assist in such a process in accordance with Cellink s instructions The Buyer is en...

Page 59: ...hs or more each Party shall be entitled to terminate the Agreement 14 CONFIDENTIALITY The Parties hereby undertake during the term of this Agreement and thereafter to hold in confidence and absolute s...

Page 60: ...General Terms shall be interpreted and construed in accordance with the laws of Virginia The United Nations Convention on Contracts for the International Sale of Goods shall not apply Any dispute con...

Page 61: ...INK holds all right title and interest in and to the CELLINK 3D Bioprinter Software Licensed Software and all associated websites platforms and applications and is the sole and exclusive owner of http...

Page 62: ...e agrees to download and install any and all updates when available and acknowledges that if not updated as recommended the Licensed Software may not operate as expected Licensee acknowledges and agre...

Page 63: ...icensee agrees that CELLINK is not responsible or liable in any way for any of Licensee s information or content CELLINK and its service providers may use access and retain Licensee s information or c...

Page 64: ...AND CELLINK 3D BIOPRINTER IS AT LICENSEE S OWNRISK CELLINKANDITSSUPPLIERSHEREBYDISCLAIMALLWARRANTIES EXPRESS IMPLIED OR STATUTORY INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY...

Page 65: ...CES FILES AND DATA AND DAMAGE TO LICENSEE S OR A THIRD PARTY S PROPERTY VIRUSES HARMFUL CODE OR OTHER HARMFUL COMPONENTS INCOMPATIBILITY WITH SOFTWARE SYSTEMS OR SERVICES INACCURACIES INCOMPLETENESS C...

Page 66: ...TE RESULTING FROM PRINTING ANY DESIGN USING THE CELLINK 3D BIOPRINTER AND OR LICENSED SOFTWARE 12 INDEMNITY Licensee agrees to indemnify defend and hold harmless CELLINK its subsidiaries officers dire...

Page 67: ...in a writing signed by CELLINK Any other modification to this User License must be made by written instrument duly executed by the Parties This User License and its formation operation and performance...

Page 68: ...that a customer has already been through all these previous options stated and is still not satisfied with the results the customer must have the bioprinter sent back at their own expense Once a retu...

Page 69: ...tial 3 months warranty starting on the purchase date Components not included in the warranty include timing belts linear ball bearings rods and the build platform Moving parts although of the highest...

Page 70: ...ermined in calculation with the FedEx tracking information Limitations This guarantee and limited warranty are sole and final as they stand in reference to BIO X CELLINK and it s products where permit...

Page 71: ......

Page 72: ...Printhead manuals...

Page 73: ...his to securely fasten the cartridge in the printhead Nozzle or needle Printhead contact The connector between BIO X and the intelligent printhead Magnet The magnet allows for securely fastening the p...

Page 74: ...pply in order to function Do not forget to connect the pressure hose to the pressure connector on the printhead holder when inserting the printhead in BIO X 1 2 3 Attach the pressure hose to the cartr...

Page 75: ...Imagination is the beginning of creation Youimaginewhatyoudesire you will what you imagine and at last you create what you will George Bernard Shaw Happy 3D bioprinting...

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