Cardinal Health CHB-AGS Instructions For Use Manual Download Page 7

Single Slide Automated Gram Stainer User Manual

7

Art specifications

:

IFU/Manual - 8"Wx10"H

Do not alter art or size.

All art prints PMS 185 Red and 

100% Black.

AMS1800062
Rev. 0  2018-06

2.0  WORK AREA REQUIREMENTS

IMPORTANT!

The Single Slide Automated Gram Stainer unit requires a level counter top surface of a minimum of 12 inches wide by 13 inches deep with a height clearance of 16 
inches. The Gram Stain Kit (Cat. No. CHB-AGS-SK) and a container filled with deionized water should be positioned at the same level as the instrument immediately 
on the left side connected by the tubing coming out of the machine. Do not place reagents below the instrument.

The Single Slide Automated Gram Stainer has one tube that drains all of the waste fluids. The Single Slide Automated Gram Stainer unit drain line can be placed 
down a drain or into another disposal container to accommodate the waste fluids according to your facility and local regulations. 

The Single Slide Automated Gram Stainer consumes less than 400 watts of power and uses an external power supply module that is supplied with 47- 63 Hz, 
100-240V, 8-4A line power. The unit requires power from a grounded outlet. The Single Slide Automated Gram Stainer overvoltage category is II, the transient 
overvoltage is 1440 volts, and the pollution category is 2. The IP protection is IP20.

The Single Slide Automated Gram Stainer is installed with tubing sets to connect the instrument to the reagents. The reagent supply line is equipped with  
a cannula (rigid tube) to immerse in the reagent supply container. Labels attached to those lines indicate which reagent is to be used with each line.  
The line labeled as DI Water needs to be immersed in the water supply container to supply the machine with available water.

3.0  RECEIVING AND UNPACKING

Unpacking and set-up assistance for the Single Slide Automated Gram Stainer may be obtained by calling the Cardinal Health™ Technical Support Department 
serviced by QuickSlide™ at (800) 266-2222 (ext. 2) during the hours of 8a.m. – 5p.m, Pacific Time, Monday through Friday. 

The external power supply is a Class I supply that must be connected to an earthed (grounded) main power outlet. Failure to connect the Single Slide Automated 
Gram Stainer as specified will prevent the electrical safety protection features to function as designed.

The power supply is equipped with one attached power cord and one removable power cord. The attached power cord is equipped with a connector that 
plugs into the Single Slide Automated Gram Stainer rear panel receptacle near the power switch. The removable power cord adapts the IEC style connector 
on the power supply to the local standard AC outlet. The cord supplied by Cardinal Health™ is appropriate for the country of operation.

4.0  MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies, such as Gram’s Iodine, Stabilized Dropper Bottle (Cat. No. CHB-GS-DI), Microscope Slide Charged Adhesion Frosted 25mmX75mm 
1mm (Cat. No. M6143), Cuvette Swabs (Cat. No. CHB-GS-CS), and Gram Stain QC Slides (Cat. No. CHB-GS-QC), Gram Stain Kit (Cat. No. CHB-AGS-SK),  
System Cleaner (Cat. No. CHB-AGS-SC), and Replacement Tubing (Cat. No. CHB-AGS-RT) 

5.0 GRAM STAIN KITS

5.1 General Information

The Single Slide Automated Gram Stainer accomplishes automatic Gram staining by a systematically staining, rinsing, decolorizing, and counter-staining the 
provided biological specimen. It is critical for the success of this automated process that the unit utilizes the Gram Stain Kit (Cat. No. CHB-AGS-SK)  
from Cardinal Health™.

NOTE:

 For quality control purposes, Gram Stain Kits are labeled with a Kit Number, Lot Number, and Expiration Date. These values are used to track and identify the 

Gram Stain Kit used in the unit, and are entered into the system as part of the Gram Stain Kit Registration procedure as explained in section 8.1.

5.2 Ordering Information

The Single Slide Automated Gram Stainer software monitors consumption of the Gram Stain Kit. When starting each Stain Cycle, the system displays a count of 
the remaining number of stain cycles available in the current kit. This remaining count should be carefully monitored so that fresh reagents may be ordered and 
available when needed.

5.3 Gram Stain Kit Installation

The Single Slide Automated Gram Stainer reagents are supplied to the unit through tubes connected to the individual reagent containers. Each tube is clearly 
labeled with a color coded tag, and the tube cannula must be inserted into the correct reagent container. Tubes are also provided for connection to a deionized 
water container and to a waste fluid drain. Use care to ensure that these lines are properly connected before use of the Single Slide Automated Gram Stain unit.

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Summary of Contents for CHB-AGS

Page 1: ...Wx10 H Do not alter art or size All art prints PMS 185 Red and 100 Black AMS1800062 Rev 0 2018 06 CHB AGS Cardinal Health Single Slide Automated Gram Stainer Instructions for Use Manual CHBAGS_GramSt...

Page 2: ...Reagent Kit Installation 7 6 0 OPERATIONS INSTRUCTIONS 8 6 1 Instrument Anatomy 8 6 2 Specimen Slide Preparation 8 6 3 Instrument Preparation 9 6 4 Stain Operation 10 7 0 QUALITY CONTROL 12 7 1 Qualit...

Page 3: ...nce Summary 18 10 0 IDLE DELAYS 18 10 1 Setting the Sleep Delay 19 11 0 UNIT SHUT DOWN 19 11 1 Unit Shut Down Instructions 19 12 0 SERVICES AND SUPPLIES 20 13 0 SAFETY DATA SHEETS 20 14 0 REFERENCES 2...

Page 4: ...th its quality management system which complies with the U S Food and Drug Administration s FDA s Quality Systems Regulation QSR and current Good Manufacturing Practices cGMP contained in Title 21 Par...

Page 5: ...7 63Hz Temperature Ambient Operation 15 30C Humidity 0 to 95 without condensation Operator Adjustments Individual adjustable stain times on both short and long cycles Adjustable sleep mode Adjustable...

Page 6: ...in of the Gram s method consists of staining with crystal violet which is then fixed with iodine The microorganisms that retain the crystal violet iodine complex appear purple under microscopic examin...

Page 7: ...afety protection features to function as designed The power supply is equipped with one attached power cord and one removable power cord The attached power cord is equipped with a connector that plugs...

Page 8: ...also not recommended Methanol fixed slides have been shown to retain two to ten times as many cells than with heat fixation 1 In addition Gram positive bacteria are much less likely to become over de...

Page 9: ...tions vary according to jurisdiction Please dispose of waste according to your facility and local regulations 6 3 4 Confirm that deionized water is present in the supply container and make sure that t...

Page 10: ...4 5 Select a Stain Delay Option Select one of the two stain delays 1 Gram Stain SHORT or 2 Gram Stain LONG by pressing the 1 or 2 key on the key pad to choose the desired stain delay times for the run...

Page 11: ...the Run Menu 7 0 QUALITY CONTROL 7 1 Quality Control Checks The quality and validity of the Gram stain process of the Single Slide Automated Gram Stainer may be periodically verified by staining a sl...

Page 12: ...iration Date into the appropriate fields as it reads on the new reagent kit label 8 1 12 Press on the key pad to select Accept and save the kit registration information 8 1 13 Press on the key pad onc...

Page 13: ...New Pump Replacement Tube Kit 8 3 1 From the Main Menu Press 2 on the key pad to select SET UP 8 3 2 Press 2 on the key pad to select Consumables 8 3 3 Press 2 on the key pad to select Review Tubing 8...

Page 14: ...ry month or 600 cycles whichever occurs first The unit runs a 10 bleach solution through the reagent and water lines to clean the internal surface of the tubing and to prevent debris build up in the w...

Page 15: ...the key pad to select Prime 9 2 23 Press on the key pad to return to the Main Menu 9 2 24 Press 1 on the key pad to select Run 9 2 25 Press 1 on the key pad to select GRAM STAIN 9 2 26 Press 2 on the...

Page 16: ...f the unit 9 3 6 Press 1 on the key pad to select Scrub The unit will automatically prime the system cleaner into the reagent lines and then begin the scrub sequence 9 3 7 During the scrub sequence us...

Page 17: ...the water cannula from the water container and place it onto a fresh paper towel or alcohol swab 9 6 2 Fill the water container with fresh deionized water and mix in the drops of Gram s Iodine 9 6 3...

Page 18: ...y a complete prime of the reagent lines This prevents reagent evaporation in the supply lines and maintains the unit in a ready state without wasting reagents as each of the reagents is expelled back...

Page 19: ...press any key to continue NOTE For the Scrub procedure it is recommended that the System Cleaner for Single Slide Automated Gram Stainer Cat No CHB AGS SC is used Alternatively decolorizer can be used...

Page 20: ...x 16 fl oz CHB GS QC QC Slides 50 pack CHB AGS SC System Cleaner 2 x 500ml CHB AGS RT Replacement Tubing Kit 8 tubes kit CHB GS CS Cuvette Cleaning Swabs 100swabs kit CHB GS DI Gram s Iodine for DI Wa...

Page 21: ...by Cardinal Health viii Use of microscope slides other than the chemically treated Cardinal Health Microscope Slide Charged Adhesion Frosted 25mm x 75mm 1mm Cat No M6143 or Hardy Diagnostic PROBond C...

Page 22: ...every 20 days 20 slides or every 8 days whichever comes first Every 15 days or every 600 slides whichever occurs first 6 Months or every 1 100 cycles whichever occurs first DeionizedWaterand IodineDi...

Page 23: ...tem trained Trainee s Initials _____ Specimen Slide Preparation Section 6 2 _____ Loading Slides Section 6 4 6 _____ Initiating a Gram Stain Section 6 4 _____ Register Reagent Kit Section 8 1 _____ Ge...

Page 24: ...2018 06 CHB AGS Distributed by Cardinal Health Waukegan IL 60085 USA 2018 06 cardinalhealth com 2018 Cardinal Health All Rights Reserved CARDINAL HEALTH and the Cardinal Health LOGO are trademarks of...

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