Cardinal Health CHB-AGS Instructions For Use Manual Download Page 17

Single Slide Automated Gram Stainer User Manual

17

Art specifications

:

IFU/Manual - 8"Wx10"H

Do not alter art or size.

All art prints PMS 185 Red and 

100% Black.

AMS1800062
Rev. 0  2018-06

9.4 Cuvette Swabbing

The cuvette may build up stain residue over time. To clean it, use a foam cleaning swab (Cat. No. CHB-GS-CS) soaked in alcohol to scrub the walls of the cuvettes. 
This should be done while the instrument is not in Run Mode, but while a Scrub Cycle is in process. Scrub the cuvette walls with a clean foam swab while 
decolorizer and water is rinsing the chamber. 

Note:

 Be careful not to accidentally overflow the cuvette with the submerged swab when performing the wash cycle.

9.5 Deionized Water Disinfectant

In order to prevent contamination, the deionized water must have stabilized gram’s iodine disinfectant (Cat. No. CHB-GS-DI) added to the container following this 
schedule, whichever comes first:

1.

 Every time the water container is refilled with deionized water.

2.

 Every 20 days of operation.

This is completed by adding 10 drops of disinfectant per gallon of deionized water into the water container.

9.6 Refilling the Water Supply

The water can be refilled at any time before the staining process has begun if the water supply is getting low. 

NOTE:

 The new water supply must be disinfected using iodine before continuing use, refer to section 9.5 above. 

To refill the water supply, do the following:

9.6.1

 Remove the water cannula from the water container and place it onto a fresh paper towel or alcohol swab.

9.6.2

 Fill the water container with fresh deionized water and mix in the drops of Gram’s Iodine.

9.6.3

 Sanitize the water cannula using an alcohol swab and place it back into the water container and screw the lid on tightly.

Note:

 When removing the water cannula, if a column of air is sucked into the cannula, an error message can occur. In order to avoid possible sensor errors caused 

by air entering into the water cannula, replace the water in the water container before staining a slide. 

Note:

 If a separate sterile water container is available, it can be used to pour more deionized water into the original water container without removing the cannula. 

Be sure to add the drops of Gram’s Iodine into the separate sterile water container full of deionized water, before pouring it into the original water container.

9.7 Maintenance Summary

Frequency

Maintenance Procedures

Every time the deionized water is refilled (or every 20 days)

• Supplement the fresh deionized water with drops of  
  

Gram’s Iodine disinfectant

 (see Section 9.5).

Monthly

Every 600 slides

• Run a 

Bleach Cycle

 (see Section 9.2).

Every 8 Days

Every 20 slides

• Run a 

Wash Cycle

 (see Section 9.1).

Every 15 days

Every 600 slides

• Run a 

Scrub Cycle

 (see Section 9.3).

• 

Swab the cuvette

 during the Scrub Cycle (see Section 9.4).

Six Months

Every 1,100 slides

• Replace the orange 

pump tubes

 (see Section 8.2).

Please utilize the Maintenance Logs to make sure these periodic tasks are completed as scheduled. See section 16.0.

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Summary of Contents for CHB-AGS

Page 1: ...Wx10 H Do not alter art or size All art prints PMS 185 Red and 100 Black AMS1800062 Rev 0 2018 06 CHB AGS Cardinal Health Single Slide Automated Gram Stainer Instructions for Use Manual CHBAGS_GramSt...

Page 2: ...Reagent Kit Installation 7 6 0 OPERATIONS INSTRUCTIONS 8 6 1 Instrument Anatomy 8 6 2 Specimen Slide Preparation 8 6 3 Instrument Preparation 9 6 4 Stain Operation 10 7 0 QUALITY CONTROL 12 7 1 Qualit...

Page 3: ...nce Summary 18 10 0 IDLE DELAYS 18 10 1 Setting the Sleep Delay 19 11 0 UNIT SHUT DOWN 19 11 1 Unit Shut Down Instructions 19 12 0 SERVICES AND SUPPLIES 20 13 0 SAFETY DATA SHEETS 20 14 0 REFERENCES 2...

Page 4: ...th its quality management system which complies with the U S Food and Drug Administration s FDA s Quality Systems Regulation QSR and current Good Manufacturing Practices cGMP contained in Title 21 Par...

Page 5: ...7 63Hz Temperature Ambient Operation 15 30C Humidity 0 to 95 without condensation Operator Adjustments Individual adjustable stain times on both short and long cycles Adjustable sleep mode Adjustable...

Page 6: ...in of the Gram s method consists of staining with crystal violet which is then fixed with iodine The microorganisms that retain the crystal violet iodine complex appear purple under microscopic examin...

Page 7: ...afety protection features to function as designed The power supply is equipped with one attached power cord and one removable power cord The attached power cord is equipped with a connector that plugs...

Page 8: ...also not recommended Methanol fixed slides have been shown to retain two to ten times as many cells than with heat fixation 1 In addition Gram positive bacteria are much less likely to become over de...

Page 9: ...tions vary according to jurisdiction Please dispose of waste according to your facility and local regulations 6 3 4 Confirm that deionized water is present in the supply container and make sure that t...

Page 10: ...4 5 Select a Stain Delay Option Select one of the two stain delays 1 Gram Stain SHORT or 2 Gram Stain LONG by pressing the 1 or 2 key on the key pad to choose the desired stain delay times for the run...

Page 11: ...the Run Menu 7 0 QUALITY CONTROL 7 1 Quality Control Checks The quality and validity of the Gram stain process of the Single Slide Automated Gram Stainer may be periodically verified by staining a sl...

Page 12: ...iration Date into the appropriate fields as it reads on the new reagent kit label 8 1 12 Press on the key pad to select Accept and save the kit registration information 8 1 13 Press on the key pad onc...

Page 13: ...New Pump Replacement Tube Kit 8 3 1 From the Main Menu Press 2 on the key pad to select SET UP 8 3 2 Press 2 on the key pad to select Consumables 8 3 3 Press 2 on the key pad to select Review Tubing 8...

Page 14: ...ry month or 600 cycles whichever occurs first The unit runs a 10 bleach solution through the reagent and water lines to clean the internal surface of the tubing and to prevent debris build up in the w...

Page 15: ...the key pad to select Prime 9 2 23 Press on the key pad to return to the Main Menu 9 2 24 Press 1 on the key pad to select Run 9 2 25 Press 1 on the key pad to select GRAM STAIN 9 2 26 Press 2 on the...

Page 16: ...f the unit 9 3 6 Press 1 on the key pad to select Scrub The unit will automatically prime the system cleaner into the reagent lines and then begin the scrub sequence 9 3 7 During the scrub sequence us...

Page 17: ...the water cannula from the water container and place it onto a fresh paper towel or alcohol swab 9 6 2 Fill the water container with fresh deionized water and mix in the drops of Gram s Iodine 9 6 3...

Page 18: ...y a complete prime of the reagent lines This prevents reagent evaporation in the supply lines and maintains the unit in a ready state without wasting reagents as each of the reagents is expelled back...

Page 19: ...press any key to continue NOTE For the Scrub procedure it is recommended that the System Cleaner for Single Slide Automated Gram Stainer Cat No CHB AGS SC is used Alternatively decolorizer can be used...

Page 20: ...x 16 fl oz CHB GS QC QC Slides 50 pack CHB AGS SC System Cleaner 2 x 500ml CHB AGS RT Replacement Tubing Kit 8 tubes kit CHB GS CS Cuvette Cleaning Swabs 100swabs kit CHB GS DI Gram s Iodine for DI Wa...

Page 21: ...by Cardinal Health viii Use of microscope slides other than the chemically treated Cardinal Health Microscope Slide Charged Adhesion Frosted 25mm x 75mm 1mm Cat No M6143 or Hardy Diagnostic PROBond C...

Page 22: ...every 20 days 20 slides or every 8 days whichever comes first Every 15 days or every 600 slides whichever occurs first 6 Months or every 1 100 cycles whichever occurs first DeionizedWaterand IodineDi...

Page 23: ...tem trained Trainee s Initials _____ Specimen Slide Preparation Section 6 2 _____ Loading Slides Section 6 4 6 _____ Initiating a Gram Stain Section 6 4 _____ Register Reagent Kit Section 8 1 _____ Ge...

Page 24: ...2018 06 CHB AGS Distributed by Cardinal Health Waukegan IL 60085 USA 2018 06 cardinalhealth com 2018 Cardinal Health All Rights Reserved CARDINAL HEALTH and the Cardinal Health LOGO are trademarks of...

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