13
Start, Monitor, and Control a Purification
Empty a Rack
For manually selected racks:
»
When a rack is removed from the system, it also
has to be cleared from the software. This is done by
selecting
None
from the
Select Rack
drop-down list.
For RFID-identified racks
R
:
»
When a rack that has been used in previous run(s) is
removed from the system and then reinserted, it is
automatically cleared and enabled for the run in progress.
»
When a rack that has been used for the run in
progress is removed from the system and then
reinserted, it is not cleared automatically. To clear
the rack, press
Empty
in the
Rack
panel.
Rack B has been used by the run in
progress and Rack A was used in a
previous run or runs.
When replacing all vessels with empty
ones, press
Empty
.
When replacing the rack with another
type of rack, select the correct type from
the
Select Rack
drop-down list.
When removing the rack and leaving the
rack position empty, select
None
from
the
Select Rack
drop-down list.
Figure 28.
Unload and empty a filled manual rack on a paused system.
Rack has been used by the run
in progress.
Rack has been removed.
Rack has been reinserted. If all vessels
are empty, press
Empty
.
Rack was used in a previous run or runs.
When removed and then reinserted, it is
automatically emptied in the software.
Figure 29.
Unload and empty a filled Selekt rack on a paused system.
R
Rack has been removed.
Rack reinserted before the run is
resumed. Collection will be resumed
in the vessel with thicker border. If all
vessels are empty, press
Empty
.
Rack reinserted after the run was
resumed. Empty vessels cannot be used.
If all vessels are empty, press
Empty
.
The rack can then be reused.
Figure 30.
Unload and replace a Selekt rack currently in use.
R
Resume the Run
Before you resume the run, ensure that the setup in the
Rack
panel corresponds to what is loaded onto the collection bed.
The system uses the assigned racks in alphabetical order in
regard to their positions on the tray. Collection will be resumed
in the vessel that is highlighted with a thicker border in the
Rack
panel; see Figure 31.
Figure 31.
The Rack panel on a system with RFID
R
. Rack A is used in
the current run but has been removed, and rack B is assigned to the run
but has not yet been used. If the run is resumed without rack A being in
position, the collection will be resumed in rack B.
Manually Fractionate
At any time during the gradient run, you can switch to a new
collection vessel by pressing in the chromatogram.
Line Flush, Decompression,
and Detector Flush
At the end of a run, i.e. after the gradient purification stage
is completed, the system performs a line flush (if enabled),
system decompression (purge), and a detector flush (if
enabled). To enable or disable flushes and specify whether
enabled flushes are collected or not, see page 24.
Auto Extend
If the collection criteria are still met (any signal is above
threshold level) when the system reaches the end of a
purification, the system extends the gradient purification
stage of the run with 25% of the total gradient length using
the final conditions of the run.
Summary of Contents for Selekt Enkel
Page 1: ...Biotage Selekt Enkel User Manual...
Page 42: ...40 Biotage Selekt Enkel User Manual Biotage 2022 Notes Notes...
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