Biotage Selekt Enkel User Manual Download

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General Information

General Information

Consumables and Accessories

Only genuine Biotage consumables and accessories must be
used in the system. To order consumables and accessories,
see contact information on the back of this document or visit
our website www.biotage.com.

Software

Part No.

Description

Qty

SEL-SPK

Biotage

Spektra Software Package

Accessories

Part No.

Description

Qty

417538SP

Column Holder Sfär 5g/10g with O-ring

417539SP

Column Holder Sfär 25g with O-ring

415343SP

Column Holder Sfär 50/100g

415337SP

Column Holder Sfär 200/350g

416047SP

Selekt Fraction Collector Tray

416301SP

13 x 100 mm RFID Rack (holds 48 tubes)

416302SP

16 x 100 mm RFID Rack (holds 35 tubes)

416303SP

16 x 150 mm RFID Rack (holds 35 tubes)

416305SP

18 x 150 mm RFID Rack (holds 28 tubes)

416306SP

25 x 150 mm RFID Rack (holds 15 tubes)

416307SP

120 mL Flask RFID Rack (holds 6 flasks)

416230SP

240 mL Flask RFID Rack (holds 10 flasks)

416225SP

480 mL Flask RFID Rack (holds 8 flasks)

416321SP

Biotage

Selekt Secondary Solvent Containment

with Biotage

Solvent Detector

416410SP

Biotage

Selekt Instrument Tray with Biotage

Solvent Detector

418405SP

Biotage

Selekt Instrument Tray with Biotage

Solvent Detector, Extended Bed

417535SP

Biotage

Selekt Solvent Detector Adapter Cable

for three Biotage

Solvent Detector

417115SP

Biotage

Safety Valve Kit

412422

Biotage

SNAP 750g/1500g Holder Assembly

412537

Male Luer Outlet Fitting for Biotage

SNAP 750g

and 1500g

412358

Biotage

SNAP XL Fitting, Female Luer,

Assembly

416331SP

Flow Cell Selekt

415115SP

Inlet Check Valve for Pump Version I

417440SP

Inlet Check Valve for Pump Version II

413245SP

Fraction Collector Needle

411916SP

Fuse 4.0 TA/250 VAC, 5 x 20 mm

413008SP

Four Solvent Inlet Lines and Filters (S1-S4)

Part No.

Description

Qty

413214

Tube, Solvent Inlet S1

413215

Tube, Solvent Inlet S2

413216

Tube, Solvent Inlet S3

413217

Tube, Solvent Inlet S4

411851SP

Waste Outlet Tube

416220SP

Selekt Tube, Front Column Inlet 40 cm

416015SP

Selekt Tube, Column Inlet 80 cm

416389SP

Selekt Tube, Column Outlet 40 cm

Hardware Upgrade Kits

Part No.

Description

Qty

417501SP

Biotage

Selekt Enkel Network Upgrade Kit

417502SP

Biotage

Selekt Enkel Extended Bed Upgrade Kit 1

417504SP

Biotage

Selekt Enkel RFID Upgrade Kit

Power Cable

Part No.

Description

Qty

418362SP

Mains China Cable

418361SP

Mains India Cable

416406SP Mains Swiss Cable

416276SP

Mains EU Cable

416277SP

Mains UK Cable

416278SP

Mains US Cable

For a complete list, please visit our website www.biotage.com.

Manufacturer

Biotage Sweden AB

Biotage Sweden AB

Box 8
SE-751 03 Uppsala
SWEDEN

Visiting address: Vimpelgatan 5

Phone: +46 18 56 59 00
Fax: +46 18 59 19 22
E-mail: [email protected]
Website: www.biotage.com

Please contact your local Biotage representative. See contact
information on the back of this document or visit our website
www.biotage.com.

Summary of Contents for Selekt Enkel

Page 1: ...Biotage Selekt Enkel User Manual...

Page 2: ...nload the Run 15 Flush the System 15 Shut Down the System 16 Results 16 Search the Results 17 Analysis of the Result 18 Customize the Report 18 Add a Report Note 19 Export the Report 19 Base a New Run...

Page 3: ...columns have a QR code that can be read by the QR reader underneath the touch screen see Figure 2 When scanning a column using the QR reader the system will be able to trace all runs performed on the...

Page 4: ...n on screen notification when a solvent level is below 20 of the set capacity Issue an on screen notification when the waste level is above 85 of the set capacity Pause the system when it is time to r...

Page 5: ...test tube or bottle reaches the specified vessel volume or a user defined maximum fraction volume for the run At any time you can manually switch to a new collection vessel by pressing in the chromat...

Page 6: ...for collection and fractionation UV Baseline Correction When the UV baseline correction option is turned on the gradient run is preceded by a light absorbance detection phase During this phase the lig...

Page 7: ...d supplied by Biotage Prepare the System Start Up the System Turn on the system using the power switch located on the left side of the system see Figure 13 Figure 13 The power switch is located on the...

Page 8: ...purification and fill them with new solvents Note Both solvent inlets must be primed with solvent to achieve the specified pump performance Note We recommend to have both solvent reservoirs on the sa...

Page 9: ...n the Rack panel or automatically identified by RFID when placed on the collection bedR See more in Specify the Rack Parameters on page 10 6 Set up the run parameters as described in the following sec...

Page 10: ...nol and ethanol very volatile modifiers such as diethyl ether or modifiers that permanently alter the properties of the silica Reversed phase TLC is not supported Slight variations in migration rates...

Page 11: ...see Figure 21 If the column has been scanned the column s ID and the number of times it has been used is also displayed For more information see Administrate Column Types on page 22 Figure 21 The Colu...

Page 12: ...ne in the corresponding Select Rack drop down list or clear the check box in front of the rack Rack Sharing The system offers the possibility to have the first fraction s of a run collected in the las...

Page 13: ...nt and waste monitoring is enabled you will be notified if there is not enough solvent or waste capacity for the run when you press 3 When the Sample Load dialog opens load your sample 4 Start the gra...

Page 14: ...es a Spektra software license Baseline Flush After baseline detection the system is flushed with the solvent mix used at the start of the gradient Gradient The system is running a purification Line Fl...

Page 15: ...will be resumed in the vessel with thicker border If all vessels are empty press Empty Rack reinserted after the run was resumed Empty vessels cannot be used If all vessels are empty press Empty The...

Page 16: ...age 9 All changes except for changes to the gradient are instant When editing the gradient the modified gradient is purple and the ongoing gradient is black see Figure 32 The modified purple gradient...

Page 17: ...lumn inlet and outlet tubes together Unload a Purification Flush the System If the last purification of the day is performed with a halogenated solvent e g DCM we recommend that you assign methanol or...

Page 18: ...using the QR reader the system will be able to trace all runs performed using a specific column based on its ID To see all runs that have been performed using a column 1 Scan the column 2 Expand the...

Page 19: ...olume handle is positioned is displayed above the handle If the gradient is shown the solvent mix is also displayed Select a collection vessel in the rack purple border and the volume handle in the ch...

Page 20: ...mize a report by selecting it in the Results view and pressing Report Setup In the Report Setup view see Figure 42 the following content can be enabled or disabled in the report The changes are shown...

Page 21: ...ct whether to export the full Selekt Enkel report or a customized version by turning the Apply Report Setup option off or on To customize the report see page 18 3 Press Export 4 In the Export dialog p...

Page 22: ...ematic of the flow paths in Figure 45 2 Ensure that the correct column is chosen The column in the current setup will be chosen in the Column Type field If you want to change this select the column mo...

Page 23: ...olume is the recommended volume for the selected column if one is selected 3 Press Flush 4 After the flush press Purge if needed to release any pressure remaining in the system Purge Use the purge fea...

Page 24: ...ssure The safety pressure in bar or psi see page 24 If reached the flow rate will be reduced to keep the pressure at this level Equilibration Pressure The maximum pressure during the equilibration in...

Page 25: ...ystem Owner and the password 1234 Before you log out it is strongly recommended that this password is changed User Parameters Visible If enabled the user account is available for use Name The user nam...

Page 26: ...0 When the waste level is at 95 of the set capacity the system will be paused and the user will be prompted to empty the waste reservoir Figure 50 85 20 Level notification for waste and solvent Valley...

Page 27: ...ing UV Zero Optional Detectors Biotage Solvent Detector Enable this option when the optional solvent detector s from Biotage is connected to the system Leak detection using up to three solvent detecto...

Page 28: ...the screen turn off the system The power switch is located on the left side of the system 8 Turn on the system Note When the system is connected to your network the used IP address is displayed in the...

Page 29: ...use a subfolder located in the share folder by adding it to the path e g server123 Biotage Selekt where server123 is the host name Biotage is the name of the share folder and Selekt is a subfolder In...

Page 30: ...g Menu and then System Settings 3 Enable the E mail option in the Network field 4 Press the corresponding Settings button and enter the required e mail settings see Figure 54 Please contact your IT de...

Page 31: ...onfirm Backup dialog opens Start the backup to the USB memory device by pressing Yes 6 When a message saying that the backup has been completed appears remove the USB memory device if applicable and p...

Page 32: ...damage electronic components Note Avoid harsh cleaners and chemicals and moisture getting into the system 1 Shut down the system as described on page 15 2 Disconnect the power cord from the power out...

Page 33: ...e the highlighted section in Figure 58 b Close the retaining nut Tip At the last couple of turns hold the flow cell flat against the retaining nut while closing the nut completely Note The retaining n...

Page 34: ...nufacturer s safety handling storage and disposal recommendations refer to the safety data sheets SDS Electrical equipment can introduce ignition hazards Ensure that all solvent manufacturers recommen...

Page 35: ...slide out the two brackets at the rear of the system see Figure 65 Reassemble by reversing the procedure Figure 64 B B A A Secondary solvent containment with a solvent detector A cable locking plate a...

Page 36: ...holder Replace the Needle 1 Shut down the system as described on page 15 2 Remove the needle 3 Assemble the new needle ferrule and peek nut P N 413245SP Ensure that the needle is flush with the end o...

Page 37: ...l not be registered as no light is reaching the detector at those particular wavelengths If using a system with a Spektra software license try performing the run with the UV Baseline Correction option...

Page 38: ...n acceptable level before proceeding with the run using the lower flow rate Restart after power failure or shutdown with overpressure Warning When releasing a stuck check valve there is a risk of a sm...

Page 39: ...eliver solvent The problem may be due to No or insufficient solvent in the reservoir s Refill the solvent reservoir s and ensure that both solvent inlet lines are submerged in solvent see Prime the So...

Page 40: ...0 04 0 210 1 5 103 4 Dichloromethane DCM 75 09 2 0 42 5 V 235 6 9 475 3 Ethanol 64 17 5 0 88 2 II 210 1 3 90 0 Ethyl acetate 141 78 6 0 58 6 VIa 255 1 4 98 3 n Heptane 142 82 5 0 01 0 210 0 7 47 4 n...

Page 41: ...t 1 412422 Biotage SNAP 750g 1500g Holder Assembly 1 412537 Male Luer Outlet Fitting for Biotage SNAP 750g and 1500g 1 412358 Biotage SNAP XL Fitting Female Luer Assembly 1 416331SP Flow Cell Selekt 1...

Page 42: ...40 Biotage Selekt Enkel User Manual Biotage 2022 Notes Notes...

Page 43: ......

Page 44: ...art Number 417510 C 2022 Biotage All rights reserved No material may be reproduced or published without the written permission of Biotage Information in this document is subject to change without noti...

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