Biotage Selekt Enkel, User Manual

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20 Biotage
®
Selekt Enkel User Manual | © Biotage 2022
Flushes and Purge
Flushes and Purge
Warning
»
Never flush the system without a column mounted or the column
inlet and outlet tubing coupled together.
»
Always use the purge feature to release any remaining pressure
in the system after a flush operation.
»
To prevent leakage, visually check all tubes and fittings before
flushing the system.
Enter the
Flushes and Purge
view (see Figure 44) by pressing
Menu and then Flushes and Purge .
Figure 44. The Flushes and Purge view.
Flush
Use the flush feature to for example:
»
Clean the flow path. If the last purification of the day
is performed with a halogenated solvent (e.g. DCM),
we recommend that you assign methanol or a similar
solvent to the inlet line used with the halogenated
solvent (see page 5) and flush with at least 30 mL.
»
Check for leaks in the tubing and fittings.
To flush the system:
1. Select the path to be flushed from the Path drop-down
menu. To flush the flow path except column channel,
select the Bypass option. To flush the column channel,
select the channel option. See a schematic of the flow
paths in Figure 45.
2. Ensure that the correct column is chosen. The column in
the current setup will be chosen in the Column Type field.
If you want to change this, select the column mounted from
the
Column Type drop-down menu. If selecting “No Column”,
ensure that the column inlet and outlet tubing is coupled
together before starting a flush.
3. Enter the flush volume in the Volume text box.
4. Enter the flow rate in the Flow Rate text box. If a column is
selected, the setting will be the recommended flow rate
for that column. Note that the maximum flow rate applied
depends on the maximum pressure setting of the column
type (if used) and the maximum aspiration rate(s) for the
used solvent(s).
5. Enter the percentage to be used of each solvent connected
to the system. If two solvents are listed and you only want
to use one of them, enter “100%” for that solvent and “0%”
for the other.
6. Ensure that a sufficient quantity of each selected solvent
is present in the solvent reservoirs.
7. Ensure that the waste reservoir has sufficient capacity for
the flush.
8. Press Flush .
9. After the flush, press Purge if needed to release any
pressure remaining in the system.
Flow Paths
Figure 45
Fraction Collector UV Pump
C
Waste
Collect
S1 S2
Bypass
. The flow paths in the system.