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24

Biotage

®

 Selekt Enkel User Manual | © Biotage 2022

System Settings

System Settings

Note:

 Only users with system owner privilege can change the 

system settings. 

Enter the software’s settings view (see Figure 49) by pressing 

Menu

 and then 

System Settings

. The following system settings 

are available.

Figure 49.

 The System Settings view for a system with RFID and network 

enabled.

System

 

»

Date (yyyy-mm-dd):

 Setting the date correctly ensures 

an accurate date stamp for your purification reports.

*

 

»

Time (hh:mm:ss):

 Setting the time correctly ensures an 

accurate time stamp for your purification reports.

*

 

»

Language: 

The language to be used in the 

software’s purification mode. English is always 
used in the Data Administration, System Settings, 
and Maintenance views, and in the reports.

 

»

Pressure Unit: 

The unit to be used by the system, bar or psi.

 

»

Audible Alarm:

 If enabled, a warning will 

sound when an error has occurred.

 

»

Mouse Pointer:

 When connecting a mouse to one of the 

USB ports, you need to enable the mouse pointer.

 

»

RFID Rack Detection

R

:

 If enabled, Selekt racks 

are automatically identified by the system 
when placed on the collection bed.

*

 The system has to be restarted for the new configuration to take effect.

Purification

Note:

 Collected flushes are added to the gradient in the report.

 

»

Solvent Monitoring:

 If enabled, the system will notify the 

user when a solvent level is below 20% of its set reservoir 
capacity (see Figure 50). When 10% is left, the system will be 
paused and the user will be prompted to refill the solvent.

 

»

Waste Monitoring: 

If enabled, the system will notify the 

user when the waste level is above 85% of the set waste 
reservoir capacity (see Figure 50). When the waste level is 
at 95% of the set capacity, the system will be paused and 
the user will be prompted to empty the waste reservoir. 

Figure 50

>85%

<20%

.

 Level notification for waste and solvent.

 

»

Valley Fractionation: 

If enabled, fractionation will 

occur when a valley is detected between two peaks 
in one of the signals in the chromatogram.

 

»

Manual UV Zero: 

If enabled, it is possible to manually 

set the current UV absorbance level to zero mAU 
(milli-absorbance unit) during the gradient run. 

Note:

 The system automatically performs a UV Zero using 

the initial gradient mix before the run is started. 

 

»

Auto Extend:

 If enabled and the collection criteria are still 

met (any signal is above threshold level) when the system 
reaches the end of a purification, the system extends the 
gradient purification stage of the run with 25% of the total 
gradient length using the final conditions of the run.

 

»

Confirm Cleanup:

 If enabled, the user will get the option 

(through a popup dialog) to manually extend the run before 
the system performs the flushes that are enabled in the 
system settings and a system decompression (purge). 

 

»

UV Baseline Correction: 

Whether the baseline correction is 

performed using the initial gradient mix to the end mix A/B 
(

Standard

) or using the initial gradient mix to 100% solvent B 

(

Full

). Only available on systems with a Spektra license.

 

»

Start Collection: 

Whether collection is to be started in the 

First Empty Vessel

First Empty Row

, or 

First Empty Rack

The first two options enable rack sharing between runs. 
For more information, see “Rack Sharing” on page 10.

 

»

Isocratic Hold: 

Whether the isocratic hold is limited to run 

for a maximum of 10 CV at the time (

Limited

) or run until 

the user ends it (

Continuous

). When limited, the run is 

paused after 10 CV, enabling the user to decide whether to 
resume the isocratic hold for up to another 10 CV or end it. 

Summary of Contents for Selekt Enkel

Page 1: ...Biotage Selekt Enkel User Manual...

Page 2: ...nload the Run 15 Flush the System 15 Shut Down the System 16 Results 16 Search the Results 17 Analysis of the Result 18 Customize the Report 18 Add a Report Note 19 Export the Report 19 Base a New Run...

Page 3: ...columns have a QR code that can be read by the QR reader underneath the touch screen see Figure 2 When scanning a column using the QR reader the system will be able to trace all runs performed on the...

Page 4: ...n on screen notification when a solvent level is below 20 of the set capacity Issue an on screen notification when the waste level is above 85 of the set capacity Pause the system when it is time to r...

Page 5: ...test tube or bottle reaches the specified vessel volume or a user defined maximum fraction volume for the run At any time you can manually switch to a new collection vessel by pressing in the chromat...

Page 6: ...for collection and fractionation UV Baseline Correction When the UV baseline correction option is turned on the gradient run is preceded by a light absorbance detection phase During this phase the lig...

Page 7: ...d supplied by Biotage Prepare the System Start Up the System Turn on the system using the power switch located on the left side of the system see Figure 13 Figure 13 The power switch is located on the...

Page 8: ...purification and fill them with new solvents Note Both solvent inlets must be primed with solvent to achieve the specified pump performance Note We recommend to have both solvent reservoirs on the sa...

Page 9: ...n the Rack panel or automatically identified by RFID when placed on the collection bedR See more in Specify the Rack Parameters on page 10 6 Set up the run parameters as described in the following sec...

Page 10: ...nol and ethanol very volatile modifiers such as diethyl ether or modifiers that permanently alter the properties of the silica Reversed phase TLC is not supported Slight variations in migration rates...

Page 11: ...see Figure 21 If the column has been scanned the column s ID and the number of times it has been used is also displayed For more information see Administrate Column Types on page 22 Figure 21 The Colu...

Page 12: ...ne in the corresponding Select Rack drop down list or clear the check box in front of the rack Rack Sharing The system offers the possibility to have the first fraction s of a run collected in the las...

Page 13: ...nt and waste monitoring is enabled you will be notified if there is not enough solvent or waste capacity for the run when you press 3 When the Sample Load dialog opens load your sample 4 Start the gra...

Page 14: ...es a Spektra software license Baseline Flush After baseline detection the system is flushed with the solvent mix used at the start of the gradient Gradient The system is running a purification Line Fl...

Page 15: ...will be resumed in the vessel with thicker border If all vessels are empty press Empty Rack reinserted after the run was resumed Empty vessels cannot be used If all vessels are empty press Empty The...

Page 16: ...age 9 All changes except for changes to the gradient are instant When editing the gradient the modified gradient is purple and the ongoing gradient is black see Figure 32 The modified purple gradient...

Page 17: ...lumn inlet and outlet tubes together Unload a Purification Flush the System If the last purification of the day is performed with a halogenated solvent e g DCM we recommend that you assign methanol or...

Page 18: ...using the QR reader the system will be able to trace all runs performed using a specific column based on its ID To see all runs that have been performed using a column 1 Scan the column 2 Expand the...

Page 19: ...olume handle is positioned is displayed above the handle If the gradient is shown the solvent mix is also displayed Select a collection vessel in the rack purple border and the volume handle in the ch...

Page 20: ...mize a report by selecting it in the Results view and pressing Report Setup In the Report Setup view see Figure 42 the following content can be enabled or disabled in the report The changes are shown...

Page 21: ...ct whether to export the full Selekt Enkel report or a customized version by turning the Apply Report Setup option off or on To customize the report see page 18 3 Press Export 4 In the Export dialog p...

Page 22: ...ematic of the flow paths in Figure 45 2 Ensure that the correct column is chosen The column in the current setup will be chosen in the Column Type field If you want to change this select the column mo...

Page 23: ...olume is the recommended volume for the selected column if one is selected 3 Press Flush 4 After the flush press Purge if needed to release any pressure remaining in the system Purge Use the purge fea...

Page 24: ...ssure The safety pressure in bar or psi see page 24 If reached the flow rate will be reduced to keep the pressure at this level Equilibration Pressure The maximum pressure during the equilibration in...

Page 25: ...ystem Owner and the password 1234 Before you log out it is strongly recommended that this password is changed User Parameters Visible If enabled the user account is available for use Name The user nam...

Page 26: ...0 When the waste level is at 95 of the set capacity the system will be paused and the user will be prompted to empty the waste reservoir Figure 50 85 20 Level notification for waste and solvent Valley...

Page 27: ...ing UV Zero Optional Detectors Biotage Solvent Detector Enable this option when the optional solvent detector s from Biotage is connected to the system Leak detection using up to three solvent detecto...

Page 28: ...the screen turn off the system The power switch is located on the left side of the system 8 Turn on the system Note When the system is connected to your network the used IP address is displayed in the...

Page 29: ...use a subfolder located in the share folder by adding it to the path e g server123 Biotage Selekt where server123 is the host name Biotage is the name of the share folder and Selekt is a subfolder In...

Page 30: ...g Menu and then System Settings 3 Enable the E mail option in the Network field 4 Press the corresponding Settings button and enter the required e mail settings see Figure 54 Please contact your IT de...

Page 31: ...onfirm Backup dialog opens Start the backup to the USB memory device by pressing Yes 6 When a message saying that the backup has been completed appears remove the USB memory device if applicable and p...

Page 32: ...damage electronic components Note Avoid harsh cleaners and chemicals and moisture getting into the system 1 Shut down the system as described on page 15 2 Disconnect the power cord from the power out...

Page 33: ...e the highlighted section in Figure 58 b Close the retaining nut Tip At the last couple of turns hold the flow cell flat against the retaining nut while closing the nut completely Note The retaining n...

Page 34: ...nufacturer s safety handling storage and disposal recommendations refer to the safety data sheets SDS Electrical equipment can introduce ignition hazards Ensure that all solvent manufacturers recommen...

Page 35: ...slide out the two brackets at the rear of the system see Figure 65 Reassemble by reversing the procedure Figure 64 B B A A Secondary solvent containment with a solvent detector A cable locking plate a...

Page 36: ...holder Replace the Needle 1 Shut down the system as described on page 15 2 Remove the needle 3 Assemble the new needle ferrule and peek nut P N 413245SP Ensure that the needle is flush with the end o...

Page 37: ...l not be registered as no light is reaching the detector at those particular wavelengths If using a system with a Spektra software license try performing the run with the UV Baseline Correction option...

Page 38: ...n acceptable level before proceeding with the run using the lower flow rate Restart after power failure or shutdown with overpressure Warning When releasing a stuck check valve there is a risk of a sm...

Page 39: ...eliver solvent The problem may be due to No or insufficient solvent in the reservoir s Refill the solvent reservoir s and ensure that both solvent inlet lines are submerged in solvent see Prime the So...

Page 40: ...0 04 0 210 1 5 103 4 Dichloromethane DCM 75 09 2 0 42 5 V 235 6 9 475 3 Ethanol 64 17 5 0 88 2 II 210 1 3 90 0 Ethyl acetate 141 78 6 0 58 6 VIa 255 1 4 98 3 n Heptane 142 82 5 0 01 0 210 0 7 47 4 n...

Page 41: ...t 1 412422 Biotage SNAP 750g 1500g Holder Assembly 1 412537 Male Luer Outlet Fitting for Biotage SNAP 750g and 1500g 1 412358 Biotage SNAP XL Fitting Female Luer Assembly 1 416331SP Flow Cell Selekt 1...

Page 42: ...40 Biotage Selekt Enkel User Manual Biotage 2022 Notes Notes...

Page 43: ......

Page 44: ...art Number 417510 C 2022 Biotage All rights reserved No material may be reproduced or published without the written permission of Biotage Information in this document is subject to change without noti...

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