BiOptic Qsep1-Lite Operation Manual Download Page 21

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3. Cartridge Kit for

Qsep

Lite

™

3.1 Cartridge Type Recommendation

◆

Qualitative Cartridge Kit

Cartridge Type

Application

L1 High Resolution Cartridge Kit



sizing range: 10-1000bp



best resolutions: 2-4 bp

PCR product screening

Restriction fragment length

polymorphism (RFLP)

CHRISPR QC

L2 Standard Cartridge Kit



sizing range: 10-1000bp



best resolutions: 4-10 bp

PCR product screening

Restriction fragment length

polymorphism (RFLP)

L3 Kilobase Cartridge Kit



sizing range: 10 bp-50 kb

Genomic DNA analysis

Plasmid purification & vector cloning

analysis

High molecular weight DNA analysis

LR1 RNA Cartridge Kit

Total RNA QC

RNA analysis

◆

Quantitative Cartridge Kit

Cartridge Type

Application

LQ1 High Resolution Quantitative

Cartridge Kit



sizing range: 10-1500bp



best resolutions: 2-4 bp



quantitative range: 0.2 ng/ μl

to 50 ng/ μl input DNA

PCR product screening

Restriction fragment length polymorphism

(RFLP)

CHRISPR QC

LQ2 Standard Quantitative

Cartridge Kit

PCR product screening

Summary of Contents for Qsep1-Lite

Page 1: ...BiOptic Inc 4F No 108 3 Minquan Rd Xindian Dist New Taipei City 23141 Taiwan R O C www bioptic com tw Revolution Qsep1 Lite Operation Manual Hardware ...

Page 2: ... is prohibited without prior written permission of BiOptic Inc except as allowed under the copyright laws Qsep Series and Q Analyzer are registered trademarks of BiOptic Inc Microsoft Windows is registered trademarks of Microsoft Corporation Product and company names listed are trademarks or trade names of their respective companies Symbols of Qsep Series Symbol Description CE mark for European Co...

Page 3: ... 11 1 1 3 Qsep1 Lite Top View 12 1 2 Qsep1 Lite Specifications 12 1 3 System Requirements 13 2 Installation Instruction 14 2 1 Environment Requirements 14 2 2 Power Considerations 15 2 3 Hardware Installation 16 2 3 1 Qsep1 Lite Installation 17 2 3 1 1 System Unpacking 17 2 3 1 2 System Installation 18 3 Cartridge Kit for Qsep1 Lite 21 3 1 Cartridge Type Recommendation 21 3 2 Cartridge Specificati...

Page 4: ...1 L1 High Resolution Cartridge Kit 29 4 2 L2 Standard Cartridge Kit 32 4 3 L3 Kilobase Cartridge Kit 35 4 4 LR1 RNA Cartridge Kit 38 4 5 LQ1 High Resolution Quantitative Cartridge Kit 41 4 6 LQ2 Standard Quantitative Cartridge Kit 44 ...

Page 5: ...damage or other loss resulting from not complying or familiar with the manuals or improper operation of the devices Applications of Qsep1 Lite Genetic Profile Screening Amplified Fragment Length Polymorphisms Bacterial Viral Genotyping and Identification PCR Product Analysis Rapid Analysis of Genomic DNA Samples Low concentration of sample detection Total RNA Analysis Packing List Each Qsep1 Lite ...

Page 6: ...6 Note The Software key contains 8GB storage space Q Analyzer for Qsep1 Lite and Qsep Series Operation Manual including Hardware version and Software version ...

Page 7: ...side down 4 Store the cartridge in the calm shell container Cartridge needs to be stored in a proper environment as the instructions of cartridge unpacking guide to maintain its lifetime and quality 5 Ensure the cartridge is placed at PARK position before shutting down or disconnecting Qsep Series Failure to do so will cause dryness of the gel at the cartridge tip which will damage the cartridge 6...

Page 8: ...H non condensing Note Strongly suggest performing this step whenever you start to operate Qsep Series Warning Qsep Series are high voltage Single channel electrophoresis system Please follow the operation manual and laboratory safety guidelines for system operation Do not remove covers For operation and safety questions please contact BiOptic Inc at the official website or with your local BiOptic ...

Page 9: ...isposable gel cartridge with integrated pre programmed test method makes capillary electrophoresis experiments no longer a painful procedure that requires well trained operators No more worries about operation and human errors caused by different operators which affect the accuracy and reproducibility of the results The following sections will describe the product overview the functions of Qsep1 L...

Page 10: ...rument 1 1 1 Qsep1 Lite Front View A Indicator lamp The green light LED will flash when turn on the power switch and when connect to the software the green light LED will breath B Cartridge Door C Sample Door and Sample Tray ...

Page 11: ...2 Qsep1 Lite Back View A Power Connector B Main Power switch C Air flow Inlet Port Connect to an external pump using an 1 8 inch tube D Cable Port Connect to a computer and the front green light LED will flash ...

Page 12: ...te Top View A Cartridge door 1 2 Qsep1 Lite Specifications Detection Fluorescence Separation Voltage 1 8 kV System Weight 5 5kg Dimension 24x 21x 30 cm System type 1 channel portable system Sample capacity 1 12 samples ...

Page 13: ...System Requirements Minimum Recommended OS Windows 10 32 bit Windows 10 64 bit CPU Intel Core i5 Intel Core i7 RAM 8G RAM 16G RAM HDD 500 GB 1 TB PORT RJ45 x 1 RJ45 x 2 Screen Resolution 1280 x 800 1920 x 1080 ...

Page 14: ... installation it is recommended to leave approximately 711 mm 28 inches clear space above the counter space or table Allow approximately 76 mm 3 inches of clear space at the rear and to the side of the instrument for proper air ventilation and electrical connections Warning Do not store transport or use Qsep Series under conditions which the temperature fluctuation could cause water condensation i...

Page 15: ...WELL GSM90B12 P1M is included in the package 100 240V AC 50 60Hz The dimensions are 24cm L 21cm W 30cm H and weight of the instrument is 5 5Kg Connect the air source within the range of 50psi 65psi Note Use a surge protector outlet for both Qsep series and the computer to avoid electromagnetic interferences and damages to the inner electrical devices ...

Page 16: ...f this occurs be sure to release the condensation water before using Qsep1 Lite If you need to continuously operate Qsep1 Lite check the air pump every 12 hours to avoid the mist of condensation water damaging the devices of Qsep1 Lite Prepare a computer with Windows operation system i e Windows 10 and install the operation software Q Analyzer for Qsep1 Lite Qsep1 Lite must use ethernet cable to c...

Page 17: ...17 2 3 1 Qsep1 Lite Installation 2 3 1 1 System Unpacking ...

Page 18: ...tighten the air tube with connector and plug power cord into Qairbox Please check the Qairbox label on the power cord 2 Unpack Qsep1 Lite Plug the power cord and Ethernet cable into Qsep1 Lite Make sure the label on the power cord is Qsep1 Lite 3 Connect the other side of Ethernet cable to the operation computer ...

Page 19: ...rbox and Qsep1 Lite 5 Insert the software key to the computer Double click on Q Analyzer for Qsep1 Lite Setup exe in the Q Analyzer folder to install the software Please install the newest software 6 Follow the setup wizard to do the installation Register software key ...

Page 20: ...set the password as 8 to 12 character password and click OK 7 Double click Q Analyzer for Qsep1 Lite to open the software Computer must be connected with Qsep1 Lite by Ethernet cable or with software key inserted 8 The software will connect to the instrument automatically Qsep1 Lite is ready to use ...

Page 21: ...riction fragment length polymorphism RFLP L3 Kilobase Cartridge Kit sizing range 10 bp 50 kb Genomic DNA analysis Plasmid purification vector cloning analysis High molecular weight DNA analysis LR1 RNA Cartridge Kit Total RNA QC RNA analysis Quantitative Cartridge Kit Cartridge Type Application LQ1 High Resolution Quantitative Cartridge Kit sizing range 10 1500bp best resolutions 2 4 bp quantitati...

Page 22: ...6 months Application PCR product screening Restriction fragment length polymorphism RFLP CHRISPR QC Standard Cartridge Kit L2 Cat No C105201 L 2pcs Sample sizing range 10 1000bp L O D 1ng μl Best Resolutions 4 10bp Analysis Time 3 mins Sample number per cartridge 200 runs Required sample volume 2μl Shelf life 6 months Application sizing range 10 5000bp best resolutions 4 10 bp quantitative range 0...

Page 23: ...months Application Genomic DNA analysis Plasmid purification vector cloning analysis High molecular weight DNA analysis RNA Cartridge Kit LR1 Cat No C105210 L 2pcs L O D of total RNA 10ng μl Analysis Time 9 mins Sample number per cartridge 100 runs Required sample volume 16μl Shelf life 4 months Application Total RNA QC RNA analysis Quantitative Cartridge Kit Quantitative High Resolution Cartridge...

Page 24: ...01 LQ 2pcs Sample sizing range 10 1500bp L O D 1ng μl Quantitative range 0 2 ng μl to 50 ng μl input DNA Best Resolutions 4 10bp Analysis Time 3 mins Sample number per cartridge 200 runs Required sample volume 2μl Shelf life 6 months Application PCR product screening Restriction fragment length polymorphism RFLP Determined by PCR product which is eluted in water The concentration is input sample c...

Page 25: ...instructions below 1 Open the cartridge shell and take out the cartridge vertically 2 Insert the pin into the small hole of cartridge and press it all the way in 3 Remove the pin and clean cartridge cap and tip with tissue Make sure the cartridge is always kept in vertical position ...

Page 26: ...ace 0 2ml PCR tube 2 Click Open Sample Door and put the sample tray into Qsep1 Lite Push the sample tray to the end Position Reagent Volume ul S well Separation buffer 200 P well distilled water 50 C well distilled water 200 M well Alignment Marker Lower Marker 20 Position Reagent Volume ul S well Separation buffer 200 P well distilled water 50 C well distilled water 200 M well Alignment Marker Qu...

Page 27: ... and insert the cartridge Cartridge guiding groove must face front 5 Click Latch Please follow the instructions in Main Control Panel to do cartridge HV check and Calibration HV check The current gray line should be flat and 2 μA to pass If HV check fail please repeat HV check 2 3 times ...

Page 28: ...28 Calibration The result needs to have two peaks and the signal and noise value must pass the threshold green line DNA RNA ...

Page 29: ...illed water 200 Size marker mix Volume ul Size Marker C109200 4 Alignment marker 20 1k 16 Sample mix Volume ul Sample 2 Alignment marker 20 1k 8 Buffer and size marker mix must be placed in 0 2ml PCR tube Size marker mix can be reused within a week Please store size marker mix at 4 C Sample mix must be placed in 0 1ml tube C104252 1 Click Open Sample Door and put the sample tray into Qsep1 Lite Pu...

Page 30: ...front 4 Click Latch and the cartridge information will be displayed 5 Select method icon 6 Click the well to select sample position Size Marker must rerun every 24 hours Create Size Marker If size marker had been created within 24 hours user can choose to use the recent size marker data to do the calculation ...

Page 31: ...31 7 Confirm the analysis information and adjust the separation duration time optional Click Run to start analysis 8 View the result and get sample size bp ...

Page 32: ... Size marker mix Volume ul Size Marker C109200 4 Alignment marker 20 1k 16 Sample mix Volume ul Sample 2 Alignment marker 20 1k 8 Buffer and size marker mix must be placed in 0 2ml PCR tube Size marker mix can be reused within a week Please store size marker mix at 4 C Sample mix must be placed in 0 1ml tube C104252 1 Click Open Sample Door and put the sample tray into Qsep1 Lite Push the sample t...

Page 33: ...front 4 Click Latch and the cartridge information will be displayed 5 Select method icon 6 Click the well to select sample position Size Marker must rerun every 24 hours Create Size Marker If size marker had been created within 24 hours user can choose to use the recent size marker data to do the calculation ...

Page 34: ...34 7 Confirm the analysis information and adjust the separation duration time optional Click Run to start analysis 8 View the result and get sample size bp ...

Page 35: ...Size marker mix Volume ul Size Marker C109200 4 Alignment marker 20 1k 16 Sample mix Volume ul Sample 2 Alignment marker 20 1k 8 Buffer and size marker mix must be placed in 0 2ml PCR tube Size marker mix can be reused within a week Please store size marker mix at 4 C Sample mix must be placed in 0 1ml tube C104252 9 Click Open Sample Door and put the sample tray into Qsep1 Lite Push the sample tr...

Page 36: ...ront 12 Click Latch and the cartridge information will be displayed 13 Select method icon 14 Click the well to select sample position Size Marker must rerun every 24 hours Create Size Marker If size marker had been created within 24 hours user can choose to use the recent size marker data to do the calculation ...

Page 37: ...37 15 Confirm the analysis information and adjust the separation duration time optional Click Run to start analysis 16 View the result and get sample size bp ...

Page 38: ...r 50 C well distilled water 200 Buffer and size marker mix must be placed in 0 2ml PCR tube Size marker mix can be reused within a week Please store size marker mix at 4 C Sample mix must be placed in 0 1ml tube C104252 1 Click Open Sample Door and put the sample tray into Qsep1 Lite Push the sample tray to the end 2 Click Park to close the sample door Sample mix Volume ul Sample 16 Alignment mark...

Page 39: ...rtridge guiding groove must face front 4 Click Latch and the cartridge information will be displayed 5 Select method icon 6 Click the well to select sample position 7 Confirm the analysis information and adjust the separation duration time optional Click Run to start analysis ...

Page 40: ...40 8 View the result and self scoring RNA quality number RQN ...

Page 41: ...ter 200 Size marker mix Volume ul Size Marker C109200 4 Quantitative marker 20 1 5k 16 Sample mix Volume ul Sample 2 Alignment marker 20 1 5k 8 Buffer and size marker mix must be placed in 0 2ml PCR tube Size marker mix can be reused within a week Please store size marker mix at 4 C Sample mix must be placed in 0 1ml tube C104252 1 Click Open Sample Door and put the sample tray into Qsep1 Lite Pus...

Page 42: ...front 4 Click Latch and the cartridge information will be displayed 5 Select method icon 6 Click the well to select sample position Size Marker must rerun every 24 hours Create Size Marker If size marker had been created within 24 hours user can choose to use the recent size marker data to do the calculation ...

Page 43: ...43 7 Confirm the analysis information and adjust the separation duration time optional Click Run to start analysis 8 View the result and get sample size bp ...

Page 44: ...r 200 Size marker mix Volume ul Size Marker C109300 4 Quantitative marker 20 5k 16 Sample mix Volume ul Sample 2 Alignment marker 20 5k 8 Buffer and size marker mix must be placed in 0 2ml PCR tube Size marker mix can be reused within a week Please store size marker mix at 4 C Sample mix must be placed in 0 1ml tube C104252 1 Click Open Sample Door and put the sample tray into Qsep1 Lite Push the ...

Page 45: ...front 4 Click Latch and the cartridge information will be displayed 5 Select method icon 6 Click the well to select sample position Size Marker must rerun every 24 hours Create Size Marker If size marker had been created within 24 hours user can choose to use the recent size marker data to do the calculation ...

Page 46: ...46 7 Confirm the analysis information and adjust the separation duration time optional Click Run to start analysis 8 View the result and get sample size bp ...

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