35
4.3
L3 Kilobase Cartridge Kit
Preparation
Prepare buffer, size marker mix, and sample mix. After preparation, allocate them at
the corresponding position.
Position: Reagent
Volume (ul)
S well: Separation buffer
200
P well: distilled water
50
C well: distilled water
200
Size marker mix
Volume (ul)
Size Marker (C109200)
4
Alignment marker (20-1k)
16
Sample mix
Volume (ul)
Sample
2
Alignment marker (20-1k)
8
*Buffer and size marker mix must be placed in 0.2ml PCR tube.
*Size marker mix can be reused within a week. Please store size marker mix at 4
°
C.
*Sample mix must be placed in 0.1ml tube (C104252).
9.
Click “Open Sample Door” and put the sample tray into
Qsep
1
-Lite.
*Push the sample tray to the end.
10.
Click “Park” to close the sample door.
Operation
