DNA Engine Tetrad 2 Thermal Cycler Operations Manual
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Terminating a Run
To terminate a run before its completion, select the appropriate block from the block selec-
tion menu in the Status window and select <Stop>. Alternatively, select the <Command>
menu in the Status window, and then select <Stop> and either <All> to terminate all pro-
grams running on all blocks, or select the desired block from the drop-down list.
Pausing/Resuming a Run
To merely pause a run, select the appropriate block from the block selection menu in
the Status window and select <Pause>. Alternatively, select the <Command> menu
in the Status window, and then select <Pause/Resume> and either <Pause All> to
pause all programs running on all blocks, or select the desired block from the drop-
down list.
To resume a run, select <Resume> from the bottom of the Status window or select
<Command>, <Pause/Resume>, and either <Resume All> or select the desired block
from the drop-down list.
Skipping a Step
To skip to the next step in a running program, select <Skip> at the bottom of the
Status window. A confirmation screen will ask you to confirm the skip, select <Yes>
to skip to the next step in the protocol displayed in the Status window.
Inaccessible Features
Considerations must also be made for the compatibility of the program with the
installed Alpha units. The Run Program window will display one or more “grayed-out”
Alpha units in the Select Blocks section if there is an incompatibility with the program
in queue, or if an Alpha unit is currently unavailable due to a protocol that is already
running.
Some scenarios which may be the cause of inaccessible features or display changes include:
Block control:
A sample temperature will not be displayed.
Gradient step:
Alpha units other than 96-well Alpha units will be “grayed-out”.
Lid step:
Alpha units will be “grayed-out” if a lid step is included in the protocol, but
there is no Moto Alpha unit installed.
Lid mode:
Alpha units will be “grayed-out” if the lid mode is not set to OFF when
using a block with no lid such as a Slide Chambers Alpha unit.
When encountering an inaccessible feature or block, please review your program
with the installed Alpha units to determine if an incompatibility is present.
