Creating Programs
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2.
Gradient from 45°C to 65°C for 3 minutes
3.
Goto step 1, 29 more times
4.
An incubation at 10°C forever
5.
END
The first actual step in the protocol is the incubation at 94°C for 1 minute. We will use
the maximum rate of temperature ramping to this step, and we would like the instrument
to beep upon reaching the target temperature.
•
Position the cursor over the Lid Control Mode step and click the left
mouse button ONCE.
Double clicking will select the step for editing, which will be covered in a later
chapter. If you mistakenly select the step for editing, and the Lid Control
Mode window appears, just select <Cancel> at the bottom of the window.
Once the step is selected, any new steps that are added will be inserted after
the Lid Control Mode step.
Tip!
Before entering a new step, always select the insertion point first.
•
Select <Temp>.
The Temperature Step window presents the operator with a number of tem-
perature adjustment options. The Temperature and Time fields as well as the
Beep on Target selection option are available at the top of the window. At the
bottom, the Increment Temp, Extend Time, and Slow Ramp options are avail-
able, each with their own <Set Parameters> button. For this particular step,
the only parameters that will be set will be the temperature and the incuba-
tion time.
Note:
The Alpha units can ramp at a maximum of 3.0°C per second. If a
slower ramp speed is not entered, this will be the default.
•
Position the cursor over the Temperature field and select.
•
Enter 94 from the numeric keypad into the field.
