Troubleshooting
Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
10-3
Table 10-1: Error Messages
The error codes listed in the table below correspond to the error codes returned in
the DNA Engine Tetrad 2 cycler’s <Error Log>. The remote error codes listed corre-
spond to the error codes returned when the DNA Engine Tetrad 2 cycler is operated
in remote mode (see the <Remote> section in Chapter 8 for more information on
remote mode).
Note:
The DNA Engine Tetrad 2 software is quite sensitive to block and heat-sink
errors for these can affect accuracy and performance. When such error messages
occur, try restarting the protocol. If the message fails to reappear, proceed as usual.
Remote
Error Code
Error Code
Reason
Action
1
BO
This sample block reached a
higher temperature than was
expected.
Call (800) 4BIORAD (in the US or
Canada), or your local distributor
(outside the US or Canada).
16
BSF-CI
An irregularity was detected in the
block’s center temperature sensor.
The sensor will be checked auto-
matically during and after the run,
and you will be notified if any per-
sistent problem is found.
No action is required at this time.
19
BSF-LI
An irregularity was detected in the
block’s left temperature sensor.
The sensor will be checked auto-
matically during and after the run,
and you will be notified if any per-
sistent problem is found.
No action is required at this time.
17
BSF-RI
An irregularity was detected in the
block’s right temperature sensor.
The sensor will be checked auto-
matically during and after the run,
and you will be notified if any per-
sistent problem is found.
No action is required at this time.
4
BSF-PT
An irregularity was detected in
one or more of the block’s tem-
perature sensors. The sensor was
checked and the resultes show an
inaccuracy in block temperature
measurement. Your program was
automatically stopped.
Call (800) 4BIORAD (in the US or
Canada), or your local distributor
(outside the US or Canada).
6
HLF
The heated lid is not preheating
correctly. Your samples have
remained at room temperature, and
your program was never started.
Recover samples and call (800)
4BIORAD (in the US or Canada),
or your local distributor (outside
the US or Canada).
