DNA Engine Tetrad 2 Thermal Cycler Operations Manual
5-6
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polymerase must synthesize during the extension step increases in later cycles
because there are more template molecules, because there are fewer active poly-
merase molecules, or both. The extra time can allow synthesis to be completed.
3. Slow Ramp:
Temperature modification which allows for slower temperature
ramping than the default maximum rate of 3.0°C/sec. The minimum rate cur-
rently allowed is 0.1°C/sec. Slower ramp times than this may be achieved using
a combination of increment and goto steps. Contact Bio-Rad Technical Support
for details.
4. Beep:
Modifies a temperature step or ramp step so the instrument will beep
when the target temperature is reached.
Types of Programs
There are two types of Tetrad 2 programs, basic and advanced. Basic programming fea-
tures a graphical interface and a graphical representation of the program steps.
Advanced programming features a text-based interface and a descriptive listing of the
program steps.
Graphical Programs
Creating a basic (graphical) program is desirable if you prefer the graphical program-
ming interface and/or wish to quickly enter a program that:
•
Does not contain more than a total of six temperature and/or gradient steps.
•
Does not contain temperature or gradient steps that contain modifications
(i.e., increment temp, extend time, slow ramp, beep).
•
Does not contain more than one goto step.
•
Does not contain a temperature incubation below 0°C.
•
Does not contain a step with an incubation lasting more than 1 hour 39 minutes
and 59 seconds (99:59). Forever incubations excepted.
Basic program
Advanced program
