DNA Engine Tetrad 2 Thermal Cycler Operations Manual
5-22
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This will add a new temperature step to the protocol as step 2 with a default
temperature of 55.0°C and a duration of 30 seconds.
Note:
Steps are always added after the step that is currently selected.
•
Select the gradient step (step 1).
•
Select <Delete Step>.
The initial gradient step will be deleted, and the newly added temperature
step will be promoted to step one.
Proceed to scenario 3 for instructions on editing step parameters.
3. If the first step displayed is a temperature step, or if a temperature step was
added as indicated in scenarios 1 or 2:
•
Position the cursor (text pointer) in the temperature field of step 1 and
select by clicking once.
A flashing insertion point will appear in the temperature field. (See the
“Selecting a Step” and “Editing Step Parameters” sections on page 5-21 for
additional selection and editing options.)
•
Use the backspace key on the numeric keypad to delete the current tem-
perature if it is not 94.0°C.
•
Enter 94 from the numeric keypad.
•
Click once to accept the change.
•
Position the cursor (text pointer) in the time:minute field of step 1 and
select by clicking once.
•
Use the backspace key to delete the current value if it is not 01.
•
Enter 01 from the numeric keypad, and click once to accept the change.
•
Position the cursor (text pointer) in the time:second field of step 1 and
select by clicking once.
•
Use the backspace key to delete the current value if it is not 00.
•
Enter 00 from the numeric keypad, and click once to accept the change.
