Creating Programs
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A Lid step and the Slow Ramp and Increment Temp options are not necessary
in our example, but the steps necessary to implement them are described
below should they be needed in other protocols.
Entering a Lid Control Step
When using the Moto Alpha unit motorized lid, it may be desirable to include steps
in the Tetrad 2 program that direct the lid to open or close. This can be particularly
useful in robotic installations. To include a lid control step in a program, Select <Lid>
in the programming window. Select either Open Lid or Close Lid in the Lid Control
window that appears. For further instruction on running the Moto Alpha unit, see “The
Moto Alpha Unit,” which appears at the end of this chapter.
The Slow Ramp Option
Earlier we described the Extend Time option for a temperature step, now we will
select the Slow Ramp option (see the “Choosing a Temperature Ramping Rate” sec-
tion for additional information). Move from the programming window to the
Temperature Step window using the <Temp> button, as before. Enter the appropriate
incubation Temperature (enter 60 as an example) and Time (enter 30 seconds as an
example).
•
Select Slow Ramp.
•
Select <Set Parameters> (for the Slow Ramp option).
The Slow Ramp window appears.
•
Position the cursor over the __°C per second field and select.
•
Enter 0.5.
•
Select <OK>.
You would expect the program step as included in a protocol to appear as follows:
Incubate at 60.0°C for 00:00:30
Ramp to 60°C at 0.5°C per second
