Bio-Optica MNT Operating Instructions Manual Download Page 2

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Operating Instructions – MNT
2020-03-020.00

Summary of Contents for MNT

Page 1: ...OPERATING INSTRUCTIONS Cryostat MNT INS2500GB 2020 03 020 00...

Page 2: ...OperatingInstructions MNT 2020 03 020 00 2...

Page 3: ...YMBOLS 4 3 SAFETY NOTES 4 4 COMPONENTS 9 5 SPECIFICATIONS 10 6 UNPACKING AND INSTALLATION 12 7 INITIAL OPERATION 16 8 OPERATION OF MICROTOME 22 9 OPERATION OF CRYOSTAT 35 10 CLEANING AND MAINTENANCE 4...

Page 4: ...posablebladesormicrotome knives Thisstopsrapiddeteriorationof thedisposable blade or microtome knife 2 SYMBOLS Dangers warnings and cautions are marked by this symbol i Special instructions regarding...

Page 5: ...n stop position before working with the knife or specimen before changing the specimen during break time Hand wheel stop released Movement Hand wheel stop activated No movement The hand wheel can be s...

Page 6: ...2020 03 020 00 6 3 2 FINGER PROTECTION Use the finger protection always Before you start any work with the knife or specimen Before changing the specimen During work break Finger protection released F...

Page 7: ...same powercircuit Before turning on the instrument check if the voltage of the mains supply is identical with the name plate of the unit 3 4 WORKING WITH KNIVES AND DISPOSABLE BLADES Microtomeknivesan...

Page 8: ...is activated The cutting motor stops immediately Emergency stop switch Fordeactivatingtheemergency stop it has to be turned then it moves automatically back into the original position 3 6 MOTORIZED O...

Page 9: ...with refrigeration system Fully automatic rotary microtome Operation manual Specimen holder 5x 22mm 5x 35 mm clear cut Brush Anti roll plate Ready to use 1x Bottle cryostat low temperature oil 4x 100...

Page 10: ...cooling Temperature rangeduring operation 10 to 25 C Temperature during storage 5 to 55 C Relative humidity max 60 non condensing Humidity during storage 60 Cryochamber Temperature range 0 C to 35 C s...

Page 11: ...in 0 5 m steps 2 20 m in 1 m steps 20 50 m in 2 m steps 50 100 m in 5 m steps Horizontal movement 28 mm Vertical movement 58 mm Retraction duringupward stroke 0 200 m free selectable definedsteps Trim...

Page 12: ...nsport Note this on the shipping documents and check the shipment for possible damage Open the cardboard box from the top and remove the accessories together with the supporting foams Then remove the...

Page 13: ...easily Handwheel must be reached easily Relative humidity lower than 60 A minimum distance between wall and rear of the instrument of 10 20 cm should be guaranteed i High room temperatures and high hu...

Page 14: ...bracket for the defrost water collector Place the defrosting water container in the metal holder and connect the defrosting water pipe and the level sensor to the device Connection of the defrost wat...

Page 15: ...o use hand wheel lever that is folded away for transport reasons Before you start any work unfold the hand wheel lever by simply pulling the lever into a horizontal position For folding the lever back...

Page 16: ...us of the quick freeze unit optional and the total electronic status ofthe instrument and a cutting counter optional TUE 13 05 05 CUT 7 m Trim 19 m Cha 20 C 22 QF 0 Status OK 0000 7 2 CONTROL PANEL So...

Page 17: ...art defrost Contrast TIME TUE 13 05 05 To move further up in the main menu list press Light Auto Light duration 10 m Chamber incr 15 C Retraction 5 m Tochangevalues selectasetting Press to open the se...

Page 18: ...iciansor instrument administrators Enter PIN 0000 Select defrosting programs 1 to 3 and set a time for the automatic defrosting programme e g each day at 12 00 h and at 23 00 h in this example Defr St...

Page 19: ...will ask for a disinfection cycle e g at 12 00 h the instrument will ask for a disinfection cycle Disinfec Req 12 00 Disin Days 1 2 _ _ _ _ Disin Delay 10 h Incr start 21 00 Disinfec Req 12 00 Select...

Page 20: ...the optional fast cooling peltier system its function can be activated or deactivated The default setting for automatic deactivation of the quick freeze is set to 30 minutes The timer for automatic d...

Page 21: ...g ON OFF If Trim latching mode is activated trimming function isactivatedanddeactivatedbypressing once If Trim latching mode is deactivated trimming functionisactivatedbycontinuouslypressing Contrast...

Page 22: ...e front of the knife holder counter clockwise Remove Insert knife Tighten the knife fixation by turning the two screws in the front of the knife holder clockwise Adjust knife height by turning the whe...

Page 23: ...E Activate hand wheel lock Removefingerprotection Loosen the blade fixation by turning the right lever counter clockwise Remove Insert blade from one side Tighten the blade fixation by turning the rig...

Page 24: ...ilting the blade holder A tilt towards the object holder increases the cutting angle clockwise turn of the knob a tilt away from the object holder decreasesthecuttingangle counter clockwise turn of th...

Page 25: ...carefully press the object on top Wait until the object is frozen mat surface or pressure 8 4 INSERTION OF OBJECT HOLDER 1 Turn hand wheel to its highest position and activate the hand wheel stop 2 Re...

Page 26: ...older counterclockwise 2 To adjust the orientation swing the right orientation screw Fix the sample orientation by turning the fixation lever on the left side of the sample holder clockwise Release th...

Page 27: ...ease the value 8 9 THICKNESS ADJUSTMENT FOR CUT Tochangethecuttingthickness press or to increase or decrease the value 8 10 TRIMMING OF SPECIMEN Insert object holder Orientatespecimenasdesired Deactiv...

Page 28: ...knessis indicated in the display e g 7 m in this example TUE 13 05 05 CUT 7 m Trim 19 m Cha 20 C 22 QF 0 Status OK 0000 Turn hand wheel until some even sections have been obtained Clean the microtome...

Page 29: ...vement of the specimen TUE 13 05 05 CUT 7 m Trim 19 m Cha 20 C 22 QF 0 40 mm s 125 mm s To adjust the setting for the downward movement press and simultaneously or to increase or decrease the speed se...

Page 30: ...Press to start the motor The footswitch can be used alternatively The LED on the left side of the button is illuminated if the motor is running Press to stop the motor The footswitch can be used alte...

Page 31: ...section 7 2 If Trim latching mode is deactivated trimming function is activated by continuously pressing Activation of trimming function is displayed as illustrated above Optionally press continuously...

Page 32: ...ad will stop at the highest position if is selected the specimen head will stop at the lowest position Press to start the motor The footswitch can be used alternatively The motor will automatically st...

Page 33: ...de or right away from blade For pick up of the samples turn the anti roll plate side wards with the help of the knob In this case make sure that the anti roll plate is folded intothe horizontal positi...

Page 34: ...tsfor individualtissues Type of Tissue Recommended temperature range at the knife or blade edge C Bone marrow 16 to 25 Brain 7 to 10 Breast with fat 25 to 30 Breast without fat 16 to 20 Cartilage 13 t...

Page 35: ...fault setting for the UV C light duration and the automatic deactivation is set to 30 minutes The timer can be set by an authorized service technician i For safety reasons UV C light will be deactivat...

Page 36: ...will be reached this place will be needed to connect the UV Lamp with the connecting plug Pic 2 3 Picture 4 The connector can be plugged into two directions of polarity a switch is excluded Picture 5...

Page 37: ...omatic shutdown of the fast freezing positions is set to 30 minutes The shutdown interval can be changed by an authorized service technician 9 3 HEAT EXTRACTOR For a more rapid freezing process with i...

Page 38: ...ressed through the evaporator All ice which has been built up is melted the water drops into the condensate bottle The defrosting process ends at a temperature of 20 C on the evaporator The chamber te...

Page 39: ...ry in order to avoid a decision Switch on the instrument i Before starting a complete defrost of the instrument assure that all samples have been removed from the cryochamber Before switching on the i...

Page 40: ...or use cleaning medium directly onto the touch panels 10 2 RECOMMENDED MAINTENANCE AND SERVICESCHEDULE Daily Remove frozen section waste from the cryostat chamber after every day of usage Disinfectio...

Page 41: ...n the blade by turning the lever counterclockwise After removing the blade pull out the lever removethepressureplateandclean the blade holder with a brush orcloth Reassemble the pressure plate andthe...

Page 42: ...technical service or spare parts are necessary please contact your local Bio Optica distributor Please have the following information available Complete contact details Type of instrument andserial nu...

Page 43: ...10159000 UV disinfection 10169000 Object Cooling 10164000 Debris extraction system 10167100 Rack for object holders 310000769 Heat extractor block 31000253 Object holder 22 mm 30000002 Object holder 2...

Page 44: ...heinstrumentismanufacturedunderan ISO 13485 2016 quality management system Unauthorized modification or repair by third party persons will void the warranty Only original Bio Optica spare parts must b...

Page 45: ......

Page 46: ...Bio Optica S p A Via San Faustino 58 20134 Milano Italy Tel 39 02 2127 131 Fax 39 02 2153 000 info bio optica it https www bio optica it...

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